LOCUS       sequence-91195-e         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E coli genetic modification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Yang J, Sun B, Huang H, Jiang Y, Diao L, Chen B, Xu C, Wang X, Liu 
            J, Jiang W, Yang S
  TITLE     High-Efficiency Scarless Genetic Modification in Escherichia coli by
            Using Lambda Red Recombination and I-SceI Cleavage.
  JOURNAL   Appl Environ Microbiol. 2014 Jul 1;80(13):3826-3834. Epub 2014 Apr 
            18.
  PUBMED    24747889
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol."; date: "2014-07-1"; volume: "80(13)"; pages: 
            "3826-3834. Epub 2014 Apr 18"
COMMENT     Sequence Label: pMDISI
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    85..118
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     oriT            125..234
                     /note="incP origin of transfer"
ORIGIN
        1 aactcgtcct cggttcccgg cgatcctctg gagattatat taccctgtta tccctaattc
       61 cggggatccg tcgacctgca gttcgaagtt cctattctct agaaagtata ggaacttcga
      121 agttcccgcc agcctcgcag agcaggattc ccgttgagca ccgccaggtg cgaataaggg
      181 acagtgaaga aggaacaccc gctcgcgggt gggcctactt cacctatcct gcccggctga
      241 cgccgttgga tacaccaagg aaagtctaca cgaacccttt ggcaaaatcc tgtatatcgt
      301 gcgaaaaagg atggatatac cgaaaaaatc gctataatga ccccgaagca gggttatgca
      361 gcggaaaatg cagctcacgg taactgatgc cgtatttgca gtaccagcgt acggcccaca
      421 gaatgatgtc acgctgaaaa tgccggcctt tgaatgggtt catgtgcagc tccataagca
      481 aaaggggatg ataagtttat caccaccgac tatttgcaac agtgccgttg atcgtgctat
      541 gatcgactga tgtcatcagc ggtggagtgc aatgtcatga gggaagcggt gatcgccgaa
      601 gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg
      661 ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt
      721 gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac
      781 gaccttttgg aaactt
//
