LOCUS       sequence-91199-e         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Reporter construct containing a 4.5 kb promoter region of the Ihh 
            gene in front of a luciferase gene for in vitro DNA transfection 
            assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Wang W, Lian N, Li L, Moss HE, Wang W, Perrien DS, Elefteriou F, 
            Yang X
  TITLE     Atf4 regulates chondrocyte proliferation and differentiation during 
            endochondral ossification by activating Ihh transcription.
  JOURNAL   Development. 2009 Dec;136(24):4143-53. doi: 10.1242/dev.043281. Epub
            2009 Nov 11.
  PUBMED    19906842
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.043281"; journalName: "Development"; date: "2009-12"; 
            volume: "136"; issue: "24"; pages: "4143-53"
COMMENT     Sequence Label: pIhh4.5k-Luc
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     78..100
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     92..109
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     93..109
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     116..135
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        116..134
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    316..450
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    570..704
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
       61 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata
      121 cgactcacta tagggcgaat tgggcccgac gtcgcatgct cctctagagc ttgcagatct
      181 gcgactctag aggatctgcg actctagagg atcataatca gccataccac atttgtagag
      241 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
      301 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
      361 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
      421 ctcatcaatg tatcttatca tgtctggatc tgcgactcta gaggatcata atcagccata
      481 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
      541 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
      601 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
      661 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatctgcgac tctagaggat
      721 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaa
//
