LOCUS       sequence-91204-e        1055 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Radeck J, Kraft K, Bartels J, Cikovic T, Durr F, Emenegger J, 
            Kelterborn S, Sauer C, Fritz G, Gebhard S, Mascher T
  TITLE     The Bacillus BioBrick Box: generation and evaluation of essential 
            genetic building blocks for standardized work with Bacillus 
            subtilis.
  JOURNAL   J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
  PUBMED    24295448
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2013-12-2"; volume: "7(1):29. doi"; pages: " 
            10.1186/1754-1611-7-29"
COMMENT     Sequence Label: PliaG
FEATURES             Location/Qualifiers
     source          1..1055
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..26
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(30..73)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    76..97
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    219..239
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      240..297
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(321..338)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(572..591)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctataaaat aggcgtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc
       61 taaggatgat ttctggaatt cgcggccgct tctagagcaa aaatcagacc agacaaaagc
      121 ggcaaatgaa taagcggaac ggggaaggat ttgcggtcaa gtccttccct tccgcacgta
      181 tcaattcgca agcttttcct ttataataga atgaatgata ctagtagcgg ccgctgcagt
      241 ccggcaaaaa agggcaaggt gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta
      301 cttcgcgtta tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
      361 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
      421 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
      481 ttgctggcgt ttttccacag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
      541 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
      601 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
      661 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
      721 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
      781 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
      841 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
      901 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
      961 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     1021 ggtagcggtg gtttttttgt ttgcaagcag cagat
//
