LOCUS       sequence-91207-e         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Very weak constitutive promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Radeck J, Kraft K, Bartels J, Cikovic T, Durr F, Emenegger J, 
            Kelterborn S, Sauer C, Fritz G, Gebhard S, Mascher T
  TITLE     The Bacillus BioBrick Box: generation and evaluation of essential 
            genetic building blocks for standardized work with Bacillus 
            subtilis.
  JOURNAL   J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
  PUBMED    24295448
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2013-12-2"; volume: "7(1):29. doi"; pages: " 
            10.1186/1754-1611-7-29"
COMMENT     Sequence Label: J23101
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     95..112
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(136..193)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    194..214
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     promoter        complement(215..249)
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
     misc_feature    250..271
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      274..317
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(321..339)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(567..586)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
       61 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcctg
      121 cataacgcga agtaatcttt tcggttttaa agaaaaaggg cagggtggtg acaccttgcc
      181 cttttttgcc ggactgcagc ggccgctact agtagctagc ataataccta ggactgagct
      241 agctgtaaac tctagaagcg gccgcgaatt ccagaaatca tccttagcga aagctaagga
      301 ttttttttat ctgaaattct gcctcgtgat acgcctattt ttataggtta atgtcatgat
      361 aataatggtt tcttagacgt caggtggcac tcgagttgat cgggcacgta agaggttcca
      421 actttcacca taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt
      481 caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat
      541 cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata
      601 accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca
      661 agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaatttc
      721 gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg
      781 ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc
      841 ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt
      901 tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttc
//
