LOCUS       sequence-91210-e        1100 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FLAG-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Radeck J, Kraft K, Bartels J, Cikovic T, Durr F, Emenegger J, 
            Kelterborn S, Sauer C, Fritz G, Gebhard S, Mascher T
  TITLE     The Bacillus BioBrick Box: generation and evaluation of essential 
            genetic building blocks for standardized work with Bacillus 
            subtilis.
  JOURNAL   J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
  PUBMED    24295448
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2013-12-2"; volume: "7(1):29. doi"; pages: " 
            10.1186/1754-1611-7-29"
COMMENT     Sequence Label: FLAG-tag
FEATURES             Location/Qualifiers
     source          1..1100
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     95..112
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(136..193)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    194..214
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     CDS             complement(224..289)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      338..381
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(385..403)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(631..650)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
       61 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcctg
      121 cataacgcga agtaatcttt tcggttttaa agaaaaaggg cagggtggtg acaccttgcc
      181 cttttttgcc ggactgcagc ggccgctact agtattaacc ggtcttgtcg tcatcgtctt
      241 tgtagtcgat atcatgatcc ttataatcac catcatgatc cttataatcg ccggccatag
      301 tagttcctcc ttatctagaa gcggccgcgg aattccagaa atcatcctta gcgaaagcta
      361 aggatttttt ttatctgaaa ttctgcctcg tgatacgcct atttttatag gttaatgtca
      421 tgataataat ggtttcttag acgtcaggtg gcactcgagt tgatcgggca cgtaagaggt
      481 tccaactttc accataatga aataagatca ctaccgggcg tattttttga gttatcgaga
      541 ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat
      601 atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc
      661 tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag
      721 cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa
      781 tttcgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac
      841 accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat
      901 ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc
      961 tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt
     1021 ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc
     1081 atgggcaaat attatacgca
//
