LOCUS       sequence-91212-e         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  10xHis-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Radeck J, Kraft K, Bartels J, Cikovic T, Durr F, Emenegger J, 
            Kelterborn S, Sauer C, Fritz G, Gebhard S, Mascher T
  TITLE     The Bacillus BioBrick Box: generation and evaluation of essential 
            genetic building blocks for standardized work with Bacillus 
            subtilis.
  JOURNAL   J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
  PUBMED    24295448
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2013-12-2"; volume: "7(1):29. doi"; pages: " 
            10.1186/1754-1611-7-29"
COMMENT     Sequence Label: His10-tag
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     95..112
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(136..193)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    194..214
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     CDS             complement(224..253)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     terminator      302..345
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(349..367)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(595..614)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
       61 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcctg
      121 cataacgcga agtaatcttt tcggttttaa agaaaaaggg cagggtggtg acaccttgcc
      181 cttttttgcc ggactgcagc ggccgctact agtattaacc ggtatgatga tgatgatgat
      241 gatgatgatg atggccggcc atagtagttc ctccttatct agaagcggcc gcggaattcc
      301 agaaatcatc cttagcgaaa gctaaggatt ttttttatct gaaattctgc ctcgtgatac
      361 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactc
      421 gagttgatcg ggcacgtaag aggttccaac tttcaccata atgaaataag atcactaccg
      481 ggcgtatttt ttgagttatc gagattttca ggagctaagg aagctaaaat ggagaaaaaa
      541 atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca
      601 tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt
      661 ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc
      721 cgcctgatga atgctcatcc ggaatttcgt atggcaatga aagacggtga gctggtgata
      781 tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg
      841 ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc
//
