LOCUS       sequence-91215-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Streptactin-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Radeck J, Kraft K, Bartels J, Cikovic T, Durr F, Emenegger J, 
            Kelterborn S, Sauer C, Fritz G, Gebhard S, Mascher T
  TITLE     The Bacillus BioBrick Box: generation and evaluation of essential 
            genetic building blocks for standardized work with Bacillus 
            subtilis.
  JOURNAL   J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
  PUBMED    24295448
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2013-12-2"; volume: "7(1):29. doi"; pages: " 
            10.1186/1754-1611-7-29"
COMMENT     Sequence Label: StrepII-tag
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     95..112
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(136..193)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    194..214
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     CDS             complement(224..247)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     terminator      296..339
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(343..361)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(589..608)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
       61 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcctg
      121 cataacgcga agtaatcttt tcggttttaa agaaaaaggg cagggtggtg acaccttgcc
      181 cttttttgcc ggactgcagc ggccgctact agtattaacc ggttttttcg aactgcgggt
      241 ggctccagcc ggccatagta gttcctcctt atctagaagc ggccgcggaa ttccagaaat
      301 catccttagc gaaagctaag gatttttttt atctgaaatt ctgcctcgtg atacgcctat
      361 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc actcgagttg
      421 atcgggcacg taagaggttc caactttcac cataatgaaa taagatcact accgggcgta
      481 ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
      541 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
      601 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
      661 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
      721 atgaatgctc atccggaatt tcgtatggca atgaaagacg gtgagctggt gatatgggat
      781 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
      841 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
      901 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
      961 gccaatccct gggtgagttt caccagtttt gatt
//
