LOCUS       sequence-91217-e         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  HA-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Radeck J, Kraft K, Bartels J, Cikovic T, Durr F, Emenegger J, 
            Kelterborn S, Sauer C, Fritz G, Gebhard S, Mascher T
  TITLE     The Bacillus BioBrick Box: generation and evaluation of essential 
            genetic building blocks for standardized work with Bacillus 
            subtilis.
  JOURNAL   J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
  PUBMED    24295448
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2013-12-2"; volume: "7(1):29. doi"; pages: " 
            10.1186/1754-1611-7-29"
COMMENT     Sequence Label: HA-tag
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     87..104
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(128..185)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    186..206
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     CDS             complement(216..242)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     terminator      291..334
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(338..356)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(584..603)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
       61 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcc tgcataacgc
      121 gaagtaatct tttcggtttt aaagaaaaag ggcagggtgg tgacaccttg cccttttttg
      181 ccggactgca gcggccgcta ctagtattaa ccggttgcat aatccggaac atcatacgga
      241 tagccggcca tagtagttcc tccttatcta gaagcggccg cggaattcca gaaatcatcc
      301 ttagcgaaag ctaaggattt tttttatctg aaattctgcc tcgtgatacg cctattttta
      361 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcactcg agttgatcgg
      421 gcacgtaaga ggttccaact ttcaccataa tgaaataaga tcactaccgg gcgtattttt
      481 tgagttatcg agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata
      541 taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt
      601 tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt
      661 aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa
      721 tgctcatccg gaatttcgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt
      781 tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga
      841 ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg
      901 tgaaaacctg gcctatttcc cctaa
//
