LOCUS       sequence-91221-e        6575 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  interacts with MAPK signaling module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6575)
  AUTHORS   Kelkar N, Gupta S, Dickens M, Davis RJ
  TITLE     Interaction of a mitogen-activated protein kinase signaling module 
            with the neuronal protein JIP3.
  JOURNAL   Mol Cell Biol. 2000 Feb;20(3):1030-43.
  PUBMED    10629060
REFERENCE   2  (bases 1 to 6575)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2000-02"; volume: "20(3)"; pages: "1030-43"
COMMENT     Sequence Label: pcdna3 T7 JIP3a
FEATURES             Location/Qualifiers
     source          1..6575
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        298..501
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     452..472
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     546..565
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        546..564
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             578..610
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     primer_bind     complement(6088..6104)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6088..6104)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(6139..6157)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6140..6157)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6177..6194)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6183..6407
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6540..6559)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
       61 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      121 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      181 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      241 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
      301 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
      361 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
      421 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
      481 gaggtctata taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc
      541 gaaattaata cgactcacta tagggagacc caagcttatg gctagcatga ctggtggaca
      601 gcaaatgggt ggatcatcga attccccgga acgagccgcg ctggcggcgg cggcggtagc
      661 cgcgatgatg gagatccaga tggacgaggg aggaggtgtg gtggtgtacc aagacgacta
      721 ctgctcgggc tcggtcatgt cggagcgtgt gtcgggcctg gcgggctcca tctaccgcga
      781 gttcgagcgc ctcattcact gctatgacga ggaggtggtc aaggagctca tgccgctggt
      841 ggtgaacgtg ctggagaacc ttgactcggt gctgagcgag aaccaggagc acgaggtgga
      901 gctggagctc ctacgcgagg acaacgagca gctgctcacg caatacgagc gcgagaaggc
      961 gctgcgcaaa caggccgagg agaaattcat cgaatttgaa gatgccttgg aacaagagaa
     1021 gaaagaactc cagatccagg tagaacatta tgagtttcag acacgccagc tggagctaaa
     1081 ggccaaaaac tatgcagatc agatttcccg actggaggaa cgagaatcgg agatgaagaa
     1141 ggaatacaat gccctgcacc agcggcacac agagatgatc cagacctatg tggaacacat
     1201 tgaaagatcc aagatgcagc aagttggggg tagcggccaa acagaaagca gcctgcccgg
     1261 gcggagcagg aaggagcgtc ccacctctct gaatgtcttc cccctggctg atggcatggt
     1321 acgtgcacag atggggggca agctcgtgcc tgcgggggac cactggcacc tgagtgacct
     1381 cggccagcta cagtccagct ccagctacca gtgtccaaac gatgagatgt ctgagtcagg
     1441 ccagtcctca gcagctgcaa cacccagtac cacaggtacc aagtccaaca cacccacgtc
     1501 ctccgtgccc tcagcagcag tcacgccact caacgagagc ctacagcccc tgggggacta
     1561 tgtcagtgtc acaaagaaca acaagcaggc ccgagagaag cgcaatagcc gtaacatgga
     1621 ggtccaggtc acccaagaga tgcggaacgt cagtatcggc atgggcagca gtgacgagtg
     1681 gtccgatgtt caggacatta tcgactccac cccagagctg gatgtgtgtc ctgaaacccg
     1741 tctggagcgc acaggaagca gcccaaccca gggaattgta aacaaagctt ttggaatcaa
     1801 cactgactcc ttgtatcacg aactctccac ggcgggatct gaggtcatcg gggatgtgga
     1861 cgagggagct gatctcctag gggagttttc aggaatgggc aaagaagtgg ggaacctgct
     1921 gctggagaac tcacagcttc tagagacaaa aaatgcttta aatgtagtga agaatgacct
     1981 cattgctaag gttgaccaac tgtcaggaga acaggaggtc ctgaagggtg agctggaagc
     2041 agccaagcaa gccaaagtca agctggagaa ccgaatcaaa gagcttgaag aagaactgaa
     2101 gagagtcaag tcagaggcag taactgcccg ccgtgagccc agagaagagg tggaggatga
     2161 caaaatcccc atggcccagc gccgacgctt cacacgggtg gagatggccc gagtgctcat
     2221 ggaacgcaac cagtacaagg aacgcctcat ggagctgcag gaggctgtga ggtggactga
     2281 aatgatcaga gcatcaaggg aacacccatc tgtccaggag aagaagaagt ccaccatctg
     2341 gcagttcttt agtcgcctct tcagctcctc atctagcccc cctccggcca aacgatccta
     2401 cccatctgtg aacattcact acaagtcacc cactgcagct ggctttagcc agcgtcgcag
     2461 ccatgctttg tgccagatct cagccggcag caggcccctg gagttcttcc ctgatgatga
     2521 ctgcacctct tctgcccggc gggagcagaa gcgggagcag taccgccagg ttcgtgaaca
     2581 cgtgcgcaat gatgacggga ggctgcaggc ctgtgggtgg agcctgcctg ccaagtacaa
     2641 gcagctgagc cccaatggag gccaggaaga cacccggatg aaaaatgtgc ctgtccctgt
     2701 gtactgtcgc cctctggtgg agaaggaccc ttcgacaaag ctgtggtgtg ctgctggtgt
     2761 caacctgagt gggtggaagc cacatgaaga ggactctagc aatggaccca agcctgtacc
     2821 aggtcgagac cctctgacct gtgaccggga aggagaaggc gaacccaaga gcacacaccc
     2881 atcacctgag aagaagaagg caaaggaaac ccctgaggca gatgctacct ccagtcgggt
     2941 atggatcctc accagcaccc tgacaaccag caaggtggtg atcattgatg ccaaccagcc
     3001 aggcacaatt gtggatcagt tcacagtctg caatgcccac gtcctgtgta tctccagcat
     3061 tcctgcggcc agtgacagtg actatccccc tggggagatg ttcctagaca gtgatgtgaa
     3121 ccctgaagat tcaggtgctg atggtgtgct ggctggcatc accctggtgg ggtgtgctac
     3181 ccgctgcaat gttccacgta gcaactgttc ctcacgagga gacaccccag tactggacaa
     3241 ggggcagggg gatgtggcga ccactgccaa tgggaaggtc aacccgtccc aatccacaga
     3301 agaagccaca gaagccacag aggtgccaga ccctggtccc agcgagtcag aagcaacgac
     3361 agtccggccc gggcctctca cagagcatgt ctttactgac ccagcaccca ccccatcctc
     3421 cagcacccag cctgccagtg agaatgggtc agaatccaat ggcaccattg tacagcctca
     3481 ggtggagccc agtggggaac tctcaacaac aaccagtagc gctgcaccca ctatgtggct
     3541 aggagcccag aatggctggc tctatgtgca ttcagcggta gccaactgga agaagtgtct
     3601 gcactccatc aagctaaaag actctgtgct gagcctggtg catgtcaaag gccgagtgct
     3661 ggtagctctt gcagatggga ccctggctat cttccatcgt ggagaggatg gccagtggga
     3721 cctgagcaac taccacctaa tggacctggg ccacccacac cactccatcc gctgcatggc
     3781 tgttgtgaat gaccgagttt ggtgtggcta caagaacaag gtgcatgtta tccagcccaa
     3841 gacaatgcag attgagaaat catttgatgc ccacccaagg cgggaaagcc aggtacgtca
     3901 gctggcctgg atcggtgatg gagtgtgggt ctctattcgc ttggattcta cccttcggct
     3961 ctaccatgct cacacccacc agcacctgca ggatgtggac attgagccct atgttagcaa
     4021 gatgctagga accggcaagc tgggcttctc cttcgtgcgc atcacagcct tactcattgc
     4081 aggcaaccgt ctgtgggtgg gcactggcaa tggggttgtc atctccatcc ccttgactga
     4141 gactgtggtc ctgcatcgag gccagctcct agggctccga gccaacaaga catccccaac
     4201 atctggggag gggacccgcc cagggggcat catccatgtg tatggggacg acagcagtga
     4261 caaggccgcc agtagtttca tcccctactg ctccatggca caggctcagc tttgcttcca
     4321 tgggcaccgt gatgctgtca aattctttgt ctctgtgcca ggaaatgtgc tggccactct
     4381 caatggcagt gtgctagaca gcccatcaga gggccctggg cctgctgcac ccgctgcaga
     4441 tgctgagggc cagaagttga agaatgcact ggtgctgagt ggtggtgaag gttacattga
     4501 cttccgtatc ggagacggag aggatgatga aactgaggaa tgtgccgggg acgtgaacca
     4561 gacaaagccc tcgttgtcca aggctgagcg cagccacatc atcgtgtggc aggtgtccta
     4621 cacccctgag tgagaccctg tcctacctga tgccaactgt acataggacc ctacctgcct
     4681 gcctccccgc ctgttccctg gggcagccag gttcgtccat ccccttttaa cctctcaact
     4741 tgcagctttt gcctgaggtc cagcccctag ctgttagaga gggatgaagc agggtttggg
     4801 gagtgagaca ggaacagatg tctttcccat cagggaggag agagcctctg agacaatctc
     4861 tccagcagcc cctggtcttg acccaacatg aagtcccagg gggccctcag ggcaaggcag
     4921 gaccaaaatg actatgggca gcccctacag gtggccctgg ctccactcca tcctcaccag
     4981 tcctggcagg agcaagagcc tcatactgcc agagtcttcc ccaagtctac ttgctttgca
     5041 tgcagccctg aggaagggct cctgatttcc aaccctttgc tctctctgag gggcccaggc
     5101 ctagccaccg tcctctgccc agaagcaagg ttagggctac cctggttccc ggggccccag
     5161 gcagccttct ctgggcttcc ccgtctccaa acttatattc ccttggcccc tagcctgggt
     5221 cttacctggg aacctggcat gaaagctcag ggccagcctc ccaagggcag ctgcttcaaa
     5281 agatgacaaa tacccgtcaa cccaagattg tccagcccct cacagaccag ttcccctccg
     5341 ctcttcctgg acacaagatt caccttggag gagcaagtgc cctgcccctc ccagaagccc
     5401 caagggtaga atttctcagg ctgccagggc aggcacaggc ctcaggaaga agggtggccc
     5461 tggtctcccc agagtcagtc ctaggatgca ggcttagcct caggttaatg gaggatggtg
     5521 tgctccaagg gcctgcctcc tgcacagggc tccaaggagc ccagctccca gacacgtact
     5581 aagtgcctag ggttgccggc tgtggcctgc tcccttggag agcagctgac aagaggcttc
     5641 caggcagagg ccagaagagc tgaggaccag ccaggatggg cacagttcta agcaagtgtg
     5701 tgggagcagg tgctgccctg gctctggcct tgctcgtcag gggcactgag tcagttggcc
     5761 tctactgcac gcttcccacc agagaagcac agatccaggg gcactgcccc tgagcctgtc
     5821 tggccacaac aagctgcagc agtgtgcagc tgcaggcttc tccccaccac cctgccacct
     5881 ctccactgtg atgtatgtcc cttgtctgtt cccacatgag tgtgacgtga ccggggttag
     5941 ttgggagggt gcaactggag ggaaggggcc agtgcaactt tcctcaggct ttggccttgc
     6001 aagtgaactc acatatctgc tctgtatgta ataaatgtct taacactaaa aaaaaaaaaa
     6061 aaaaaaagga attcctgcag cccgggggat ccactagttc tagagcggcc gctcgagcat
     6121 gcatctagag ggccctattc tatagtgtca cctaaatgct agagctcgct gatcagcctc
     6181 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     6241 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     6301 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     6361 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     6421 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     6481 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     6541 tcctttcgct ttcttccctt cctttctcgc cacgt
//
