LOCUS       sequence-91225-e         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mouse genomic ROSA26 targeting vector, expresses EGFP, U6promoter to
            drive short RNAs, pgkneo, cassette flanked by loxP sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Brown JR, Zetsche B, Jackson-Grusby L
  TITLE     RUSH and CRUSH: a rapid and conditional RNA interference method in 
            mice.
  JOURNAL   Genesis. 2014 Jan;52(1):39-48. doi: 10.1002/dvg.22718. Epub 2013 Oct
            26.
  PUBMED    24166816
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/dvg.22718";
            journalName: "Genesis"; date: "2014-01"; volume: "52"; issue: "1"; 
            pages: "39-48"
COMMENT     Sequence Label: pRUSH
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(209..274,275..312)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     regulatory      360..369
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(411..432)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(672..691)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tggagtaggc ggggagaagg ccgcaccctt ctccggaggg gggaggggag tgttgcaata
       61 cctttctggg agttctctgc tgcctcctgg cttctgagga ccgccctggg cctgggagaa
      121 tcccttcccc ctcttccctc gtgatctgca actccagtct ttctacggtc gaggctagaa
      181 ctagtggatc ccccgggctg cagatctgta gggcgcagta gtccagggtt tccttgatga
      241 tgtcatactt atcctgtccc ttttttttcc acagctcgcg gttgaggaca aactcttcgc
      301 ggtctttcca gtggggatcg acggtatcga tggatccgaa ttcagtggat ccaccggtcg
      361 ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
      421 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
      481 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
      541 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
      601 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
      661 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
      721 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aaacatcctg
      781 ggg
//
