LOCUS       sequence-91228-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mouse genomic ROSA26 targeting vector, expresses EGFP, cre/lox 
            conditional U6 promoter pgk-puro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Brown JR, Zetsche B, Jackson-Grusby L
  TITLE     RUSH and CRUSH: a rapid and conditional RNA interference method in 
            mice.
  JOURNAL   Genesis. 2014 Jan;52(1):39-48. doi: 10.1002/dvg.22718. Epub 2013 Oct
            26.
  PUBMED    24166816
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/dvg.22718";
            journalName: "Genesis"; date: "2014-01"; volume: "52"; issue: "1"; 
            pages: "39-48"
COMMENT     Sequence Label: pCRUSH
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(204..269,270..307)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     regulatory      355..364
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(361..363,364..366,367..825)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMA"
     primer_bind     complement(406..427)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(667..686)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 taggcgggga gaaggccgca cccttctccg gaggggggag gggagtgttg caataccttt
       61 ctgggagttc tctgctgcct cctggcttct gaggaccgcc ctgggcctgg gagaatccct
      121 tccccctctt ccctcgtgat ctgcaactcc agtctttcta cggtcgaggc tagaactagt
      181 ggatcccccg ggctgcagat ctgtagggcg cagtagtcca gggtttcctt gatgatgtca
      241 tacttatcct gtcccttttt tttccacagc tcgcggttga ggacaaactc ttcgcggtct
      301 ttccagtggg gatcgacggt atcgatggat ccgaattcag tggatccacc ggtcgccacc
      361 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
      421 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      481 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      541 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
      601 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      661 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      721 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      781 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaa
//
