LOCUS       sequence-91234-e         515 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of bovine pancreatic ribonuclease A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 515)
  AUTHORS   delCardayre SB, Ribo M, Yokel EM, Quirk DJ, Rutter WJ, Raines RT
  TITLE     Engineering ribonuclease A: production, purification and 
            characterization of wild-type enzyme and mutants at Gln11.
  JOURNAL   Protein Eng. 1995 Mar;8(3):261-73.
  PUBMED    7479688
REFERENCE   2  (bases 1 to 515)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Eng."; date: "1995-03"; volume: "8(3)"; pages: "261-73"
COMMENT     Sequence Label: pET22b RNase A
FEATURES             Location/Qualifiers
     source          1..515
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1..23
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     sig_peptide     31..96
                     /codon_start=1
                     /gene="pelB (fragment)"
                     /product="leader peptide for secretion"
                     /label=pelB signal sequence
                     /translation="MKYLLPTAAAGLLLLAAQPAMA"
     CDS             97..141
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 tttgtttaac tttaagaagg agatatacat atgaaatacc tgctgccgac cgctgctgct
       61 ggtctgctgc tcctcgctgc ccagccggcg atggccaagg aaactgcagc agccaagttt
      121 gagcggcagc acatggactc cagcacttcc gctgccagca gctccaacta ctgtaaccag
      181 atgatgaaga gccggaacct gaccaaagat cgatgcaagc cagtgaacac ctttgtgcac
      241 gagtccctgg ctgatgtcca ggccgtgtgc tcccagaaaa atgttgcctg caagaatggg
      301 cagaccaatt gctaccagag ctactccacc atgagcatca ccgactgccg tgagaccggc
      361 agctccaagt accccaactg tgcctacaag accacccagg cgaataaaca catcattgtg
      421 gcttgtgagg gaaacccgta cgtgccagtc cactttgatg cttcagtgta gtagtcgaca
      481 agcttgcggc cgcactcgag caccaccacc accac
//
