LOCUS       sequence-91240-e         419 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human codon-optimized S. pyogenes Cas9 from the human 
            PGK-1 gene promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Maggio I, Holkers M, Liu J, Janssen JM, Chen X, Goncalves MA
  TITLE     Adenoviral vector delivery of RNA-guided CRISPR/Cas9 nuclease 
            complexes induces targeted mutagenesis in a diverse array of human 
            cells.
  JOURNAL   Sci Rep. 2014 May 29;4:5105. doi: 10.1038/srep05105.
  PUBMED    24870050
REFERENCE   2  (bases 1 to 419)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2014-05-29"; volume: "4:5105. doi"; pages: " 
            10.1038/srep05105"
COMMENT     Sequence Label: pAdSh.PGK.Cas9
FEATURES             Location/Qualifiers
     source          1..419
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      49..58
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 caccgacctc tctccccacc gggtacgtcg ctagaggatc gaacccttgc caccatggac
       61 aagaagtact ccattgggct cgatatcggc acaaacagcg tcggctgggc cgtcattacg
      121 gacgagtaca aggtgccgag caaaaaattc aaagttctgg gcaataccga tcgccacagc
      181 ataaagaaga acctcattgg cgccctcctg ttcgactccg gggagacggc cgaagccacg
      241 cggctcaaaa gaacagcacg gcgcagatat acccgcagaa agaatcggat ctgctacctg
      301 caggagatct ttagtaatga gatggctaag gtggatgact ctttcttcca taggctggag
      361 gagtcctttt tggtggagga ggataaaaag cacgagcgcc acccaatctt tggcaatat
//
