LOCUS       sequence-91244-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  reporter plasmid containing 1 myc cDNA binding sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Gupta S, Seth A, Davis RJ
  TITLE     Transactivation of gene expression by Myc is inhibited by mutation 
            at the phosphorylation sites Thr-58 and Ser-62.
  JOURNAL   Proc Natl Acad Sci U S A. 1993 Apr 15;90(8):3216-20.
  PUBMED    8386367
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "1993-04-15"; volume: "90(8)"; pages: 
            "3216-20"
COMMENT     Sequence Label: pMyc1ElbLuc,
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(92..110)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(93..110)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(191..210)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     310..332
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(370..388)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        456..560
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(779..798)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gctaattcga gctcggtacc acattatata ccctctgcag gcatgcaagc ttagttgacc
       61 acgtggtcaa ctaagcttca gctgctcgag ttctatagtg tcacctaaat cgtatgtgta
      121 tgatacataa ggttatgtat taattgtagc cgcgttctaa cgacaatatg tccatatggt
      181 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
      241 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
      301 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
      361 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
      421 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      481 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      541 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      601 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      661 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      721 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      781 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      841 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      901 gcatgacagt aagagaatta
//
