LOCUS       sequence-91245-e         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  U6 promoter-driven single guide RNA complementary to the human AAVS1
            locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Maggio I, Holkers M, Liu J, Janssen JM, Chen X, Goncalves MA
  TITLE     Adenoviral vector delivery of RNA-guided CRISPR/Cas9 nuclease 
            complexes induces targeted mutagenesis in a diverse array of human 
            cells.
  JOURNAL   Sci Rep. 2014 May 29;4:5105. doi: 10.1038/srep05105.
  PUBMED    24870050
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2014-05-29"; volume: "4:5105. doi"; pages: " 
            10.1038/srep05105"
COMMENT     Sequence Label: pAdSh.U6.gRNAS1
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    83..107
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_RNA        complement(119..194)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(215..463)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(273..292)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(443..463)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_signal     519..669
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     repeat_region   757..859
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
ORIGIN
        1 tgctcgaagc ggccgctcga gctctgtaca tgtccgcggt cgtagtaacg gccgccagtg
       61 tgctggaatt cgccctttaa tgccaacttt gtacaagaaa gctgggtcta gaaaaaaagc
      121 accgactcgg tgccactttt tcaagttgat aacggactag ccttatttta acttgctatt
      181 tctagctcta aaacatcctg tccctagtgg ccccggtgtt tcgtcctttc cacaagatat
      241 ataaagccaa gaaatcgaaa tactttcaag ttacggtaag catatgatag tccattttaa
      301 aacataattt taaaactgca aactacccaa gaaattatta ctttctacgt cacgtatttt
      361 gtactaatat ctttgtgttt acagtcaaat taattctaat tatctctcta acagccttgt
      421 atcgtatatg caaatatgaa ggaatcatgg gaaataggcc ctcttcctgc ccgaccttgg
      481 tacgcatgca agctagatcg catgcggtac gtaccagtat tacgcgctat gagtaacaca
      541 aaattattca gatttcactt cctcttattc agttttcccg cgaaaatggc caaatcttac
      601 tcggttacgc ccaaatttac tacaacatcc gcctaaaacc gcgcgaaaat tgtcacttcc
      661 tgtgtacacc ggcgcacacc aaaaacgtca cttttgccac atccgtcgct tacatgtgtt
      721 ccgccacact tgcaacatca cacttccgcc acactactac gtcacccgcc ccgttcccac
      781 gccccgcgcc acgtcacaaa ctccaccccc tcattatcat attggcttca atccaaaata
      841 aggtatatta ttgatgatgt taattaaaga attaattcga tcctgaatgg cgaatggacg
      901 cgccctgtag cggccgcatt taaccgccgg cggggtgtgg gtggttacgc gccagcgtga
      961 cccgcta
//
