LOCUS       sequence-91246-e         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  reporter plasmid containing 2 myc cDNA binding sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Gupta S, Seth A, Davis RJ
  TITLE     Transactivation of gene expression by Myc is inhibited by mutation 
            at the phosphorylation sites Thr-58 and Ser-62.
  JOURNAL   Proc Natl Acad Sci U S A. 1993 Apr 15;90(8):3216-20.
  PUBMED    8386367
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "1993-04-15"; volume: "90(8)"; pages: 
            "3216-20"
COMMENT     Sequence Label: pMyc2ElbLuc,
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(175..193)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(176..193)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(274..293)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     393..415
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(453..471)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        539..643
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(862..881)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 atgtttttgg cgtcttccat ttggatccgg gccctctaga tgcggccgca tgcataagct
       61 aattcgagct cggtaccaca ttatataccc tctgcaggca tgcaagctta gttgaccacg
      121 tggtcagtta agcttagttg accacgtggt cagttaagct tcagctgctc gagttctata
      181 gtgtcaccta aatcgtatgt gtatgataca taaggttatg tattaattgt agccgcgttc
      241 taacgacaat atgtccatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
      301 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
      361 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
      421 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
      481 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
      541 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
      601 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
      661 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
      721 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
      781 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
      841 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
      901 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
      961 cacagaaaag catcttacgg a
//
