LOCUS       sequence-91248-e         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  wildtype Cas9 expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Tsai SQ, Wyvekens N, Khayter C, Foden JA, Thapar V, Reyon D, Goodwin
            MJ, Aryee MJ, Joung JK
  TITLE     Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome 
            editing.
  JOURNAL   Nat Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2908.
  PUBMED    24770325
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2908."
COMMENT     Sequence Label: pSQT817
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 acgactcaga aggggcaaaa aaacagtcga gagcggatga agagaataga agagggtatt
       61 aaagaactgg gcagccagat cttaaaggag catcctgtgg aaaataccca attgcagaac
      121 gagaaacttt acctctatta cctacaaaat ggaagggaca tgtatgttga tcaggaactg
      181 gacataaacc gtttatctga ttacgacgtc gatcacattg taccccaatc ctttttgaag
      241 gacgattcaa tcgacaataa agtgcttaca cgctcggata agaaccgagg gaaaagtgac
      301 aatgttccaa gcgaggaagt cgtaaagaaa atgaagaact attggcggca gctcctaaat
      361 gcgaaactga taacgcaaag aaagttcgat aacttaacta aagctgagag gggtggcttg
      421 tctgaacttg acaaggccgg atttattaaa cgtcagctcg tggaaacccg ccaaatcaca
      481 aagcatgttg cacagatact agattcccga atgaatacga aatacgacga gaacgataag
      541 ctgattcggg aagtcaaagt aatcacttta aagtcaaaat tggtgtcgga cttcagaaag
      601 gattttcaat tctataaagt tagggagata aataactacc accatgcgca cgacgcttat
      661 cttaatgccg tcgtagggac cgcactcatt aagaaatacc cgaagctaga aagtgagttt
      721 gtgtatggtg attacaaagt ttatgacgtc cgtaagatga tcgcgaaaag cgaacaggag
      781 ataggcaagg ctacagccaa atacttcttt tattctaaca ttatgaattt ctttaagacg
      841 gaaatcactc tggcaaacgg agagatacgc aaacgacctt taattgaaac caatggggag
      901 acaggtgaaa tcgtatggga ta
//
