LOCUS       sequence-91249-e         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  reporter plasmid containing 4 myc cDNA binding sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Gupta S, Seth A, Davis RJ
  TITLE     Transactivation of gene expression by Myc is inhibited by mutation 
            at the phosphorylation sites Thr-58 and Ser-62.
  JOURNAL   Proc Natl Acad Sci U S A. 1993 Apr 15;90(8):3216-20.
  PUBMED    8386367
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "1993-04-15"; volume: "90(8)"; pages: 
            "3216-20"
COMMENT     Sequence Label: pMyc4ElbLuc.
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(176..194)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(177..194)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(275..294)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     394..416
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(454..472)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        540..644
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(863..882)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gcataagcta attcgagctc ggtaccacat tatataccct ctgcaggcat gcaagcttaa
       61 ctgaccacgt ggtcaactaa gcttagttga ccacgtggtc agttaagctt agttgaccac
      121 gtggtcagtt aagcttcagt cgaccacgtg gtcgatcgac cacgtctgct cgagttctat
      181 agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg tagccgcgtt
      241 ctaacgacaa tatgtccata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
      301 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
      361 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
      421 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
      481 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
      541 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
      601 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
      661 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
      721 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
      781 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
      841 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
      901 a
//
