LOCUS       sequence-91250-e        6659 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  interacts with MAPK signaling module .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6659)
  AUTHORS   Kelkar N, Gupta S, Dickens M, Davis RJ
  TITLE     Interaction of a mitogen-activated protein kinase signaling module 
            with the neuronal protein JIP3.
  JOURNAL   Mol Cell Biol. 2000 Feb;20(3):1030-43.
  PUBMED    10629060
REFERENCE   2  (bases 1 to 6659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2000-02"; volume: "20(3)"; pages: "1030-43"
COMMENT     Sequence Label: pcdna3 Flag JIP3b
FEATURES             Location/Qualifiers
     source          1..6659
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        74..377
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        378..581
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     532..552
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             733..756
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(6222..6240)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6223..6240)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6260..6277)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6266..6490
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6623..6642)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
       61 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      121 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      181 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      241 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      301 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      361 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      421 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      481 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      541 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac
      601 ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc caagcttggt
      661 accgagctcg gatccactag taacggccgc cagtgtgctg gaattctgca gataattccg
      721 gcagccaaca tggactataa ggacgatgat gacaaaatgg agatccagat ggacgaggga
      781 ggaggtgtgg tggtgtacca agacgactac tgctcgggct cggtcatgtc ggagcgtgtg
      841 tcgggcctgg cgggctccat ctaccgcgag ttcgagcgcc tcattcactg ctatgacgag
      901 gaggtggtca aggagctcat gccgctggtg gtgaacgtgc tggagaacct tgactcggtg
      961 ctgagcgaga accaggagca cgaggtggag ctggagctcc tacgcgagga caacgagcag
     1021 ctgctcacgc aatacgagcg cgagaaggcg ctgcgcaaac aggccgagga gaaattcatc
     1081 gaatttgaag atgccttgga acaagagaag aaagaactcc agatccaggt agaacattat
     1141 gagtttcaga cacgccagct ggagctaaag gccaaaaact atgcagatca gatttcccga
     1201 ctggaggaac gagaatcgga gatgaagaag gaatacaatg ccctgcacca gcggcacaca
     1261 gagatgatcc agacctatgt ggaacacatt gaaagatcca agatgcagca agttgggggt
     1321 agcggccaaa cagaaagcag cctgcccggg cggagcagga aggagcgtcc cacctctctg
     1381 aatgtcttcc ccctggctga tggcatggta cgtgcacaga tggggggcaa gctcgtgcct
     1441 gcgggggacc actggcacct gagtgacctc ggccagctac agtccagctc cagctaccag
     1501 tgtccaaacg atgagatgtc tgagtcaggc cagtcctcag cagctgcaac acccagtacc
     1561 acaggtacca agtccaacac acccacgtcc tccgtgccct cagcagcagt cacgccactc
     1621 aacgagagcc tacagcccct gggggactat gtcagtgtca caaagaacaa caagcaggcc
     1681 cgagagaagc gcaatagccg taacatggag gtccaggtca cccaagagat gcggaacgtc
     1741 agtatcggca tgggcagcag tgacgagtgg tccgatgttc aggacattat cgactccacc
     1801 ccagagctgg atgtgtgtcc tgaaacccgt ctggagcgca caggaagcag cccaacccag
     1861 ggaattgtaa acaaagcttt tggaatcaac actgactcct tgtatcacga actctccacg
     1921 gcgggatctg aggtcatcgg ggatgtggac gagggagctg atctcctagg ggagttttca
     1981 gtgcgcgatg atttttttgg aatgggcaaa gaagtgggga acctgctgct ggagaactca
     2041 cagcttctag agacaaaaaa tgctttaaat gtagtgaaga atgacctcat tgctaaggtt
     2101 gaccaactgt caggagaaca ggaggtcctg aagggtgagc tggaagcagc caagcaagcc
     2161 aaagtcaagc tggagaaccg aatcaaagag cttgaagaag aactgaagag agtcaagtca
     2221 gaggcagtaa ctgcccgccg tgagcccaga gaagaggtgg aggatgtaag cagctatctc
     2281 tgtacagaat tggacaaaat ccccatggcc cagcgccgac gcttcacacg ggtggagatg
     2341 gcccgagtgc tcatggaacg caaccagtac aaggaacgcc tcatggagct gcaggaggct
     2401 gtgaggtgga ctgaaatgat cagagcatca agggaacacc catctgtcca ggagaagaag
     2461 aagtccacca tctggcagtt ctttagtcgc ctcttcagct cctcatctag cccccctccg
     2521 gccaaacgat cctacccatc tgtgaacatt cactacaagt cacccactgc agctggcttt
     2581 agccagcgtc gcagccatgc tttgtgccag atctcagccg gcagcaggcc cctggagttc
     2641 ttccctgatg atgactgcac ctcttctgcc cggcgggagc agaagcggga gcagtaccgc
     2701 caggttcgtg aacacgtgcg caatgatgac gggaggctgc aggcctgtgg gtggagcctg
     2761 cctgccaagt acaagcagct gagccccaat ggaggccagg aagacacccg gatgaaaaat
     2821 gtgcctgtcc ctgtgtactg tcgccctctg gtggagaagg acccttcgac aaagctgtgg
     2881 tgtgctgctg gtgtcaacct gagtgggtgg aagccacatg aagaggactc tagcaatgga
     2941 cccaagcctg taccaggtcg agaccctctg acctgtgacc gggaaggaga aggcgaaccc
     3001 aagagcacac acccatcacc tgagaagaag aaggcaaagg aaacccctga ggcagatgct
     3061 acctccagtc gggtatggat cctcaccagc accctgacaa ccagcaaggt ggtgatcatt
     3121 gatgccaacc agccaggcac aattgtggat cagttcacag tctgcaatgc ccacgtcctg
     3181 tgtatctcca gcattcctgc ggccagtgac agtgactatc cccctgggga gatgttccta
     3241 gacagtgatg tgaaccctga agattcaggt gctgatggtg tgctggctgg catcaccctg
     3301 gtggggtgtg ctacccgctg caatgttcca cgtagcaact gttcctcacg aggagacacc
     3361 ccagtactgg acaaggggca gggggatgtg gcgaccactg ccaatgggaa ggtcaacccg
     3421 tcccaatcca cagaagaagc cacagaagcc acagaggtgc cagaccctgg tcccagcgag
     3481 tcagaagcaa cgacagtccg gcccgggcct ctcacagagc atgtctttac tgacccagca
     3541 cccaccccat cctccagcac ccagcctgcc agtgagaatg ggtcagaatc caatggcacc
     3601 attgtacagc ctcaggtgga gcccagtggg gaactctcaa caacaaccag tagcgctgca
     3661 cccactatgt ggctaggagc ccagaatggc tggctctatg tgcattcagc ggtagccaac
     3721 tggaagaagt gtctgcactc catcaagcta aaagactctg tgctgagcct ggtgcatgtc
     3781 aaaggccgag tgctggtagc tcttgcagat gggaccctgg ctatcttcca tcgtggagag
     3841 gatggccagt gggacctgag caactaccac ctaatggacc tgggccaccc acaccactcc
     3901 atccgctgca tggctgttgt gaatgaccga gtttggtgtg gctacaagaa caaggtgcat
     3961 gttatccagc ccaagacaat gcagattgag aaatcatttg atgcccaccc aaggcgggaa
     4021 agccaggtac gtcagctggc ctggatcggt gatggagtgt gggtctctat tcgcttggat
     4081 tctacccttc ggctctacca tgctcacacc caccagcacc tgcaggatgt ggacattgag
     4141 ccctatgtta gcaagatgct aggaaccggc aagctgggct tctccttcgt gcgcatcaca
     4201 gccttactca ttgcaggcaa ccgtctgtgg gtgggcactg gcaatggggt tgtcatctcc
     4261 atccccttga ctgagactgt ggtcctgcat cgaggccagc tcctagggct ccgagccaac
     4321 aagacatccc caacatctgg ggaggggacc cgcccagggg gcatcatcca tgtgtatggg
     4381 gacgacagca gtgacaaggc cgccagtagt ttcatcccct actgctccat ggcacaggct
     4441 cagctttgct tccatgggca ccgtgatgct gtcaaattct ttgtctctgt gccaggaaat
     4501 gtgctggcca ctctcaatgg cagtgtgcta gacagcccat cagagggccc tgggcctgct
     4561 gcacccgctg cagatgctga gggccagaag ttgaagaatg cactggtgct gagtggtggt
     4621 gaaggttaca ttgacttccg tatcggagac ggagaggatg atgaaactga ggaatgtgcc
     4681 ggggacgtga accagacaaa gccctcgttg tccaaggctg agcgcagcca catcatcgtg
     4741 tggcaggtgt cctacacccc tgagtgagac cctgtcctac ctgatgccaa ctgtacatag
     4801 gaccctacct gcctgcctcc ccgcctgttc cctggggcag ccaggttcgt ccatcccctt
     4861 ttaacctctc aacttgcagc ttttgcctga ggtccagccc ctagctgtta gagagggatg
     4921 aagcagggtt tggggagtga ggcaggaaca gatgtctttc ccatcaggga ggagagagcc
     4981 tctgagacaa tctctccagc agcccctggt cttgacccaa catgaagtcc cagggggccc
     5041 tcagggcaag gcaggaccaa aatgactatg ggcagcccct acaggtggcc ctggctccac
     5101 tccatcctca ccagtcctgg caggagcaag agcctcatac tgccagagtc ttccccaagt
     5161 ctacttgctt tgcatgcagc cctgaggaag ggctcctgat ttccaaccct ttgctctctc
     5221 tgaggggccc aggcctagcc accgtcctct gcccagaagc aaggttaggg ctaccctggt
     5281 tcccggggcc ccaggcagcc ttctctgggc ttccccgtct ccaaacttat attcccttgg
     5341 cccctagcct gggtcttacc tgggaacctg gcatgaaagc tcagggccag cctcccaagg
     5401 gcagctgctt caaaagatga caaatacccg tcaacccaag attgtccagc ccctcacaga
     5461 ccagttcccc tccgctcttc ctggacacaa gattcacctt ggaggagcaa gtgccctgcc
     5521 cctcccagaa gccccaaggg tagaatttct caggctgcca gggcaggcac aggcctcagg
     5581 aagaagggtg gccctggtct ccccagagtc agtcctagga tgcaggctta gcctcaggtt
     5641 aatggaggat ggtgtgctcc aagggcctgc ctcctgcaca gggctccaag gagcccagct
     5701 cccagacacg tactaagtgc ctagggttgc cggctgtggc ctgctccctt ggagagcagc
     5761 tgacaagagg cttccaggca gaggccagaa gagctgagga ccagccagga tgggcacagt
     5821 tctaagcaag tgtgtgggag caggtgctgc cctggctctg gccttgctcg tcaggggcac
     5881 tgagtcagtt ggcctctact gcacgcttcc caccagagaa gcacagatcc aggggcactg
     5941 cccctgagcc tgtctggcca caacaagctg cagcagtgtg cagctgcagg cttctcccca
     6001 ccaccctgcc acctctccac tgtgatgtat gtcccttgtc tgttcccaca tgagtgtgac
     6061 gtgaccgggg ttagttggga gggtgcaact ggagggaagg ggccagtgca actttcctca
     6121 ggctttggcc ttgcaagtga actcacatat ctgctctgta tctcgtgccg aattatccat
     6181 cacactggcg gccgctcgag catgcatcta gagggcccta ttctatagtg tcacctaaat
     6241 gctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     6301 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     6361 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     6421 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     6481 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     6541 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     6601 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgtt
//
