LOCUS       sequence-91265-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector for parallel SLIC cloning. C-terminal 
            tags can be added during SLIC..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Scholz J, Besir H, Strasser C, Suppmann S
  TITLE     A new method to customize protein expression vectors for fast, 
            efficient and background free parallel cloning.
  JOURNAL   BMC Biotechnol. 2013 Feb 14;13(1):12.
  PUBMED    23410102
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2013-02-14"; volume: "13(1)"; pages: "12"
COMMENT     Sequence Label: pCoofy47
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     451..470
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             628..651
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     RBS             688..696
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 ggccagctgt tgggctcgcg gttgaggaca aactcttcgc ggtctttcca gtactcttgg
       61 atcggaaacc cgtcggcctc cgaacggtac tccgccaccg agggacctga gcgagtccgc
      121 atcgaccgga tcggaaaacc tctcgagaaa ggcgtctaac cagtcacagt cgcaaggtag
      181 gctgagcacc gtggcgggcg gcagcgggtg gcggtcgggg ttgtttctgg cggaggtgct
      241 gctgatgatg taattaaagt aggcggtctt gagacggcgg atggtcgagg tgaggtgtgg
      301 caggcttgag atccagctgt tggggtgagt actccctctc aaaagcgggc attacttctg
      361 cgctaagatt gtcagtttcc aaaaacgagg aggatttgat attcacctgg cccgatctgg
      421 ccatacactt gagtgacaat gacatccact ttgcctttct ctccacaggt gtccactccc
      481 aggtccaagt ttaaacggat ctctagcgaa ttcatgaact ttctgctgtc ctgggtgcac
      541 tggtccctgg ccctgctgct gtacctgcac cacgccaagt ggtcccaggc cgctcctatg
      601 gccgagggcg gtggccagaa cgaattcctg gaagttctgt tccaggggcc cttgacaaca
      661 taaaaacttt gtgttatact tgtaacgtaa ggaggtaatg attcagttta aggtttacac
      721 ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac
      781 gcccgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc
      841 ccgtgaactt tacccggtgg tgcatatcgg
//
