LOCUS       sequence-91269-e         762 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Pichia pastoris expression vector for parallel SLIC cloning. 
            C-terminal tags can be added during SLIC..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Scholz J, Besir H, Strasser C, Suppmann S
  TITLE     A new method to customize protein expression vectors for fast, 
            efficient and background free parallel cloning.
  JOURNAL   BMC Biotechnol. 2013 Feb 14;13(1):12.
  PUBMED    23410102
REFERENCE   2  (bases 1 to 762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2013-02-14"; volume: "13(1)"; pages: "12"
COMMENT     Sequence Label: pCoofy42
FEATURES             Location/Qualifiers
     source          1..762
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             111..119
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(156..179)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(join(191..202,203..400,401..457))
                     /codon_start=1
                     /gene="MF-alpha-1"
                     /product="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
                     /label=alpha-factor secretion signal
                     /note="Cleavage by the Kex2 protease occurs after the 
                     dibasic KR sequence. The EA dipeptides are then removed by 
                     dipeptidyl aminopeptidase A."
                     /translation="MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYSDLE
                     GDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKREAEA"
ORIGIN
        1 gggggatcac catccgtcgc ccgggcgtgt caataatatc actctgtaca tccacaaaca
       61 gacgataacg gctctctctt ttataggtgt aaaccttaaa ctgaatcatt acctccttac
      121 gttacaagta taacacaaag tttttatgtt gtcaagggcc cctggaacag aacttccagg
      181 aattcatcga tgcttcagcc tctctcttct cgagagatac cccttcttct ttagcagcaa
      241 tgctggcaat agtagtattt ataaacaata acccgttatt tgtgctgttg gaaaatggca
      301 aaacagcaac atcgaaatcc ccttctaaat ctgagtaacc gatgacagct tcagccggaa
      361 tttgtgccgt ttcatcttct gttgtagtgt tgactggagc agctaatgcg gaggatgctg
      421 cgaataaaac agcagtaaaa attgaaggaa atctcatcgt ttcgaataat tagttgtttt
      481 ttgatcttct caagttgtcg ttaaaagtcg ttaaaatcaa aagcttgtca attggaacca
      541 gtcgcaatta tgaaagtaag ctaataatga tgataaaaaa aaaggtttaa gacagggcag
      601 cttccttctg tttatatatt gctgtcaagt aggggttaga acagttaaat tttgatcatg
      661 aacgttaggc tatcagcagt attcccacca gaatcttgga agcatacaat gtggagacaa
      721 tgcataatca tccaaaaagc gggtgtttcc ccatttgcgt tt
//
