LOCUS       sequence-91270-e         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral plasmid expressing mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  TITLE     Tsoulfas Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV-mCherry
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(41..751)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(319..338)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     588..606
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      748..757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(822..846)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(843..894)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(872..892)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 atctttcaca aattttgtaa tccagaggtt gattgtcgac ttacttgtac agctcgtcca
       61 tgccgccggt ggagtggcgg ccctcggcgc gttcgtactg ttccacgatg gtgtagtcct
      121 cgttgtggga ggtgatgtcc aacttgatgt tgacgttgta ggcgccgggc agctgcacgg
      181 gcttcttggc cttgtaggtg gtcttgacct cagcgtcgta gtggccgccg tccttcagct
      241 tcagcctctg cttgatctcg cccttcaggg cgccgtcctc ggggtacatc cgctcggagg
      301 aggcctccca gcccatggtc ttcttctgca ttacggggcc gtcggagggg aagttggtgc
      361 cgcgcagctt caccttgtag atgaactcgc cgtcctgcag ggaggagtcc tgggtcacgg
      421 tcaccacgcc gccgtcctcg aagttcatca cgcgctccca cttgaagccc tcggggaagg
      481 acagcttcaa gtagtcgggg atgtcggcgg ggtgcttcac gtaggccttg gagccgtaca
      541 tgaactgagg ggacaggatg tcccaggcga agggcagggg gccacccttg gtcaccttca
      601 gcttggcggt ctgggtgccc tcgtaggggc ggccctcgcc ctcgccctcg atctcgaact
      661 cgtggccgtt cacggagccc tccatgtgca ccttgaagcg catgaactcc ttgatgatgg
      721 ccatgttatc ctcctcgccc ttgctcacca tggtggcggc ggatcctcta gagtcggtgt
      781 cttctatgga ggtcaaaaca gcgtggatgg cgtctccagg cgatctgacg gttcactaaa
      841 cgagctctgc ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaatggg
      901 gcgga
//
