LOCUS       sequence-91272-e         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression vector for parallel SLIC cloning containing 
            N-terminal 6x His and MBP tags. C-terminal tags can be added during 
            SLIC..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Scholz J, Besir H, Strasser C, Suppmann S
  TITLE     A new method to customize protein expression vectors for fast, 
            efficient and background free parallel cloning.
  JOURNAL   BMC Biotechnol. 2013 Feb 14;13(1):12.
  PUBMED    23410102
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2013-02-14"; volume: "13(1)"; pages: "12"
COMMENT     Sequence Label: pCoofy49
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             28..45
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 actttaagaa ggagatatac catgaaacat caccatcacc atcaccccat gaaaatcgaa
       61 gaaggtaaac tggtaatctg gattaacggc gataaaggct ataacggtct cgctgaagtc
      121 ggtaagaaat tcgagaaaga taccggaatt aaagtcaccg ttgagcatcc ggataaactg
      181 gaagagaaat tcccacaggt tgcggcaact ggcgatggcc ctgacattat cttctgggca
      241 cacgaccgct ttggtggcta cgctcaatct ggcctgttgg ctgaaatcac cccggacaaa
      301 gcgttccagg acaagctgta tccgtttacc tgggatgccg tacgttacaa cggcaagctg
      361 attgcttacc cgatcgctgt tgaagcgtta tcgctgattt ataacaaaga tctgctgccg
      421 aacccgccaa aaacctggga agagatcccg gcgctggata aagaactgaa agcgaaaggt
      481 aagagcgcgc tgatgttcaa cctgcaagaa ccgtacttca cctggccgct gattgctgct
      541 gacgggggtt atgcgttcaa gtatgaaaac ggcaagtacg acattaaaga cgtgggcgtg
      601 gataacgctg gcgcgaaagc gggtctgacc ttcctggttg acctgattaa aaacaaacac
      661 atgaatgcag acaccgatta ctccatcgca gaagctgcct ttaataaagg cgaaacagcg
      721 atgaccatca acggcccgtg ggcatggtcc aacatcgaca ccagcaaagt gaattatggt
      781 gtaacggtac tgccgacctt caagggtcaa cc
//
