LOCUS       sequence-91282-e         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector for parallel SLIC cloning. C-terminal 
            tags can be added during SLIC..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Scholz J, Besir H, Strasser C, Suppmann S
  TITLE     A new method to customize protein expression vectors for fast, 
            efficient and background free parallel cloning.
  JOURNAL   BMC Biotechnol. 2013 Feb 14;13(1):12.
  PUBMED    23410102
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2013-02-14"; volume: "13(1)"; pages: "12"
COMMENT     Sequence Label: pCoofy47
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             73..102
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             join(106..111,112..135,136..171,172..195)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             199..243
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             250..327
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             334..369
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
ORIGIN
        1 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg
       61 ggaatataag agcatcatca tcatcaccac caccaccacc actgaagcgc ttggagccac
      121 ccgcagttcg aaaaaggtgg aggttctggc ggtggatcgg gaggttcagc gtggagccac
      181 ccgcagttcg agaaatagaa agaaaccgct gctgctaaat tcgaacgcca gcacatggac
      241 agctagatga agcggcggtg gaagaaaaac tttatcgccg tgtccgccgc caaccggttc
      301 aagaagatca gcagctctgg cgccctgctg tgagaggacc aggtggaccc ccggctgatc
      361 gatggcaaat gaggcagcgg caagatcctg cacaaccaga acgtgaacag ctggggcccc
      421 atcaccgtga cccctaccac cgacggcggc gagacaagat tcgacggcca gatcatcgtg
      481 cagatggaaa acgaccccgt ggtggccaag gccgctgcca atctggccgg caagcacgcc
      541 gagagcagcg tggtggtgca gctggacagc gacggcaact accgggtggt gtacggcgac
      601 cccagcaagc tggacggcaa gctgcggtgg cagctggtgg gacacggcag ggaccacagc
      661 gagacaaaca acacccggct gagcggctac agcgccgacg agctggccgt gaagctggcc
      721 aagttccagc agagcttcaa ccaggccgag aacatcaaca acaagcccga ccacatcagc
      781 atcgtgggct gcagcctggt gtccgacgac aagcagaagg gct
//
