LOCUS       sequence-91283-e         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector for parallel SLIC cloning containing 
            N-terminal HA, 8x His and FLAG tags. C-terminal tags can be added 
            during SLIC..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Scholz J, Besir H, Strasser C, Suppmann S
  TITLE     A new method to customize protein expression vectors for fast, 
            efficient and background free parallel cloning.
  JOURNAL   BMC Biotechnol. 2013 Feb 14;13(1):12.
  PUBMED    23410102
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2013-02-14"; volume: "13(1)"; pages: "12"
COMMENT     Sequence Label: pCoofy50
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             74..103
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     polyA_signal    208..602
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
ORIGIN
        1 agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg
       61 gggaatataa gagcatcatc atcatcacca ccaccaccac cactgagcgg ccgctttcga
      121 atctagaaat tcaccccacc agtgcaggct gcctatcaga aagtggtggc tggtgtggct
      181 aatgccctgg cccacaagta tcactaagct cgctttcttg ctgtccaatt tctattaaag
      241 gttcctttgt tccctaagtc caactactaa actgggggat attatgaagg gccttgagca
      301 tctggattct gcctaataaa aaacatttat tttcattgca atgatgtatt taaattattt
      361 ctgaatattt tactaaaaag ggaatgtggg aggtcagtgc atttaaaaca taaagaaatg
      421 aagagctagt tcaaaccttg ggaaaataca ctatatctta aactccatga aagaaggtga
      481 ggctgcaaac agctaatgca cattggcaac agcccctgat gcctatgcct tattcatccc
      541 tcagaaaagg attcaagtag aggcttgatt tggaggttaa agttttgcta tgctgtattt
      601 tacattactt attgttttag ctgtcctcat gaatgtcttt tcactaccca tttgcttatc
      661 ctgcatctct cagccttgac tccactcagt tctcttgctt agagatacca cctttcccct
      721 gaagtgttcc ttccatgttt tacggcgaga tggtttctcc tcgcctggcc actcagcc
//
