LOCUS       sequence-91285-e         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Pichia pastoris expression vector for parallel SLIC cloning. 
            C-terminal tags can be added during SLIC..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Scholz J, Besir H, Strasser C, Suppmann S
  TITLE     A new method to customize protein expression vectors for fast, 
            efficient and background free parallel cloning.
  JOURNAL   BMC Biotechnol. 2013 Feb 14;13(1):12.
  PUBMED    23410102
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2013-02-14"; volume: "13(1)"; pages: "12"
COMMENT     Sequence Label: pCoofy42
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             73..102
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             join(106..111,112..135,136..171,172..195)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             199..243
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             250..327
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             332..367
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
ORIGIN
        1 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg
       61 ggaatataag agcatcatca tcatcaccac caccaccacc actgaagcgc ttggagccac
      121 ccgcagttcg aaaaaggtgg aggttctggc ggtggatcgg gaggttcagc gtggagccac
      181 ccgcagttcg agaaatagaa agaaaccgct gctgctaaat tcgaacgcca gcacatggac
      241 agctagatga agcggcggtg gaagaaaaac tttatcgccg tgtccgccgc caaccggttc
      301 aagaagatca gcagctctgg cgccctgctg agaggaccag gtggaccccc ggctgatcga
      361 cggcaaatga ggcagcggca agatcctgca caaccagaac gtgaacagct ggggccccat
      421 caccgtgacc cctaccaccg acggcggcga gacaagattc gacggccaga tcatcgtgca
      481 gatggaaaac gaccccgtgg tggccaaggc cgctgccaat ctggccggca agcacgccga
      541 gagcagcgtg gtggtgcagc tggacagcga cggcaactac cgggtggtgt acggcgaccc
      601 cagcaagctg gacggcaagc tgcggtggca gctggtggga cacggcaggg accacagcga
      661 gacaaacaac acccggctga gcggctacag cgccgacgag ctggccgtga agctggccaa
      721 gttccagcag agcttcaacc aggccgagaa catcaacaac aagcccgacc acatcagcat
      781 cgtgggctgc agcctggtgt ccgacgacaa gcagaagggc ttcggccacc ag
//
