LOCUS       sequence-91286-e         979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependent activation of gene of interest by removing Stop 
            sequences; Puro selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Jackson EL, Willis N, Mercer K, Bronson RT, Crowley D, Montoya R, 
            Jacks T, Tuveson DA
  TITLE     Analysis of lung tumor initiation and progression using conditional 
            expression of oncogenic K-ras.
  JOURNAL   Genes Dev. 2001 Dec 15. 15(24):3243-8.
  PUBMED    11751630
REFERENCE   2  (bases 1 to 979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2001 Dec 15. 15(24):3243-8."
COMMENT     Sequence Label: Lox-Stop-Lox TOPO
FEATURES             Location/Qualifiers
     source          1..979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..22
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3..21
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(67..100)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(724..744)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 tgtaatacga ctcactatag ggcgaattgg gccctctaga tgcatgctcg agcggccgct
       61 gtcgacataa cttcgtatag catacattat acgaagttat ctcgaccagc ttctgatgga
      121 attagaactt ggcaaaacaa tactgagaat gaagtgtatg tggaacagag gctgctgatc
      181 tcgttcttca ggctatgaaa ctgacacatt tggaaaccac agtacttaga accacaaagt
      241 gggaatcaag agaaaaacaa tgatcccacg agagatctat agatctatag atcatgagtg
      301 ggaggaatga gctggccctt aatttggttt tgcttgttta aattatgata tccaactatg
      361 aaacattatc ataaagcaat agtaaagagc cttcagtaaa gagcnggcat ttatctaatc
      421 ccaccccacc cccacccccg tagctccaat ccttccattc aaaatgtagg tactctgttc
      481 tcacccttct taacaaagta tgacaggaaa aacttccatt ttagtggaca tctttattgt
      541 ttaatagatc atcaatttct gcatcccggg gatctgatat catcgatgca tggggtcgtg
      601 cgctcctttc ggtcgggcgc tgcgggtcgt ggggcgggcg tcaggcaccg ggcttgcggg
      661 tcatgcacca ggtgcgcggt ccttcgggca cctcgacgtc ggcggtgacg gtgaagccga
      721 gccgctcgta gaaggggagg ttgcggggcg cggaggtctc caggaaggcg ggcaccccgg
      781 cgcgctcggc cgcctccact ccggggagca cgacggcgct gcccagaccc ttgccctggt
      841 ggtcgggcga gacgccgacg gtggccagga accacgcggg ctccttgggc cggtgcggcg
      901 ccaggaggcc ttccatctgt tgctgcgcgg ccagccggga accgctcaac tcggccatgc
      961 gcgggccgat ctcggcgaa
//
