LOCUS       sequence-91295-e        6124 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  labels macrophages with mcherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6124)
  TITLE     mpeg1-mcherry
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: tol2-mpeg1-mcherry
FEATURES             Location/Qualifiers
     source          1..6124
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(561..572,573..710)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     regulatory      2604..2613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2610..3320
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2755..2773)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3023..3042
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    3443..3564
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3480..3499)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3534..3553
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    join(3686..3873,3874..3885)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(3940..3960)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3940..3958)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3979..3995)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3979..3995)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3992..4014)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4003..4019
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4027..4033,4034..4051,4052..4057))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4072..4093
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4210..4227)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4381..4969)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4461..4480)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5140..5931,5932..6000))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5763..5782
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6001..6105)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggccac aatgacggct actacatggt
      361 gccattcctt cctctttata ggaatggaga ctacctcctg tccaacaagg ctcttggata
      421 cgagtacgcc tacctgttgg acccaggtca ttgcacaaca ccagaaatgc cctctgatct
      481 gcaaaagacg tgaatatctg ttcagacacc catatccact ctgttccaca caggtcagag
      541 gtttgtccag gagttcttga cagaggtgta aaaagtactc aaaaatttta ctcaagtgaa
      601 agtacaagta cttagggaaa attttactca attaaaagta aaagtatctg gctagaatct
      661 tacttgagta aaagtaaaaa agtactccat taaaattgta cttgagtatt aaggaagtaa
      721 aaggctagca agatctgctc gagtgttgga gcacatctga catctgaaat tatttgaaag
      781 cagcagaaat gtttttacag ctattacaaa atttacttaa caagaatcct ttaaaatatg
      841 acaatttatt gccaataatt tacagtaagt tttgcagtac tttaatatta caaattatac
      901 aactgtgctg gcacacaaaa aataacttaa tccagcacaa caacaacaac aacaactaca
      961 aaaagcatat ttccttttgc tttttcttgt tcactcaact tttggaaaca tcaactgcac
     1021 tgacctaaaa ttatacagta tgtcagtaat acaaaaacac ttagttaggt taacataaga
     1081 ttattagttt tctggagagg tgaactactc tgtaagaaac ctttttaagt tgtgccaact
     1141 aaacattttt atcagcagaa ctacaatgct tgatgagtga acaaaaaaac aaacaaacct
     1201 tatttccttt tgttttttct tgtgtactta acattttttt cacctttatt tgaaacgaca
     1261 gtagggagta tggacaggaa agtgttggga gaggagaggg atgagcatag aaccaggagg
     1321 taggaatcaa actcaggtca ctatgaacac cggagtgcca ggtgtcaaca cactaaccac
     1381 tacaccatca actccaacat ttacttgaaa acatcagttg agtgaacaaa aaactcttgt
     1441 ggaaattggt actgagaaat aatacattat cacaacaaac aaaacagaga caaatgttaa
     1501 caaaacaatt attagaaaca gaattagaaa ccggacagga ggtaaaacaa taatgataaa
     1561 caagacaagg atcaaaacat ggcaggacaa agacagtttt ttgttcactc aacctcagat
     1621 attttaggtc agtgcaactg atgtttacaa aggttgtatg catgtcaaca ccaatggtgt
     1681 agtgattagt gtgttgacac aaggcactcc agtgttcaca ctgatgctgg tttgattccc
     1741 acatcgatgt cctttgctaa tcctgcctct ctccccatgc tttcctgtct atactctcta
     1801 ctgttgtgtc aattaaaggt gaaaaaagtt aagcacaaaa gaaaaatcta aggatataag
     1861 gattatgttt ttttttgttc actcaaaatc aggcattcca gttctgcaga caaaaaatgt
     1921 tcagttggca caacttaaaa agttttgtta cagagtagtt cacctctcca gaaaactaat
     1981 aatcttatgt taacccaact aaatgttttt actgaaaaat aattgaatgt cagtgcagct
     2041 gatgtttttt taaaagtgga gagaacaaaa aaagtgaaag gaaataagga ttcattgttg
     2101 taccgactta tttttttgaa gaaaattctg ctatagggct ttcatgatcc cctatgctca
     2161 cagcagtttt aggagatgct tataataaag aggatttgaa aaacctgcta atcttgtaca
     2221 tttctaaaac aagaagccaa ccaatttcaa ttcctcctct ttcaaagcca atggaaaatt
     2281 taaaacgcac agtctcttgc gtcatcaaaa ccattctgaa gactcatcaa aatccttcgt
     2341 agctttaaat ataaatctat tattgaacag atcatacttc acaaaaaata taaatatatt
     2401 tgattcattc acttcatgaa attgtccttc aacttaacgt ttgaacgcaa ggtaccaaaa
     2461 cacaaagagg aacctcatag tctcatgacc cttcccccac ggatttgaaa ctgagaagtg
     2521 gttttattga ggaactcagc aaaatctata atcaccatat ttgattgaaa atcacattag
     2581 tgcaggagac agcaaaagga tccgccacca tggtgagcaa gggcgaggag gataacatgg
     2641 ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg
     2701 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
     2761 tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca
     2821 tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt
     2881 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga
     2941 ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg
     3001 gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
     3061 cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga
     3121 agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc
     3181 ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg
     3241 aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca
     3301 tggacgagct gtacaagtaa agcggccgcg actctagatc ataatcagcc ataccacatt
     3361 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     3421 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     3481 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     3541 gtccaaactc atcaatgtat cttaaggcgt aaattgtaag cgttaatatt ttgttaaaat
     3601 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcgagatcc
     3661 tgcggccgca agggaaaata gaatgaagtg atctccaaaa aataagtact ttttgactgt
     3721 aaataaaatt gtaaggagta aaaagtactt ttttttctaa aaaaatgtaa ttaagtaaaa
     3781 gtaaaagtat tgatttttaa ttgtactcaa gtaaagtaaa aatccccaaa aataatactt
     3841 aagtacagta atcaagtaaa attactcaag tactttacac ctctggttct tgacccccta
     3901 ccttcagcaa gcccagcaga tccactagat cttttgttcc ctttagtgag ggttaattgc
     3961 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     4021 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     4081 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     4141 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     4201 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     4261 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     4321 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     4381 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     4441 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     4501 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     4561 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     4621 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     4681 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     4741 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     4801 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     4861 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     4921 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     4981 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     5041 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     5101 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     5161 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     5221 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     5281 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     5341 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     5401 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     5461 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     5521 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     5581 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     5641 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     5701 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     5761 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     5821 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     5881 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     5941 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     6001 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     6061 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     6121 agtg
//
