LOCUS       sequence-91296-e         697 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression vector for parallel SLIC cloning containing 
            N-terminal 6x His and GST tags. C-terminal tags can be added during 
            SLIC..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Scholz J, Besir H, Strasser C, Suppmann S
  TITLE     A new method to customize protein expression vectors for fast, 
            efficient and background free parallel cloning.
  JOURNAL   BMC Biotechnol. 2013 Feb 14;13(1):12.
  PUBMED    23410102
REFERENCE   2  (bases 1 to 697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2013-02-14"; volume: "13(1)"; pages: "12"
COMMENT     Sequence Label: pCoofy48
FEATURES             Location/Qualifiers
     source          1..697
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(5..22)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctcagtggtg gtggtggtgg tgctcgagtg cggccgctca ttccaggccc tgggcgtccc
       61 agctcaggga caccttgttg ttttcggcct tctgcaccca gtcgccattg gcgtccttgg
      121 tgtgctttct gccggcctcg tccacggcca gttcgctaga ccgcacggac acgtccaccc
      181 gcaggccgtt ggcgtccata gcgttgatga actggtggcc gaagcccttc tgcttgtcgt
      241 cggacaccag gctgcagccc acgatgctga tgtggtcggg cttgttgttg atgttctcgg
      301 cctggttgaa gctctgctgg aacttggcca gcttcacggc cagctcgtcg gcgctgtagc
      361 cgctcagccg ggtgttgttt gtctcgctgt ggtccctgcc gtgtcccacc agctgccacc
      421 gcagcttgcc gtccagcttg ctggggtcgc cgtacaccac ccggtagttg ccgtcgctgt
      481 ccagctgcac caccacgctg ctctcggcgt gcttgccggc cagattggca gcggccttgg
      541 ccaccacggg gtcgttttcc atctgcacga tgatctggcc gtcgaatctt gtctcgccgc
      601 cgtcggtggt aggggtcacg gtgatggggc cccagctgtt cacgttctgg ttgtgcagga
      661 tcttgccgct gcctcatttg ccatcgatca gccgggg
//
