LOCUS       sequence-91297-e         735 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression vector for parallel SLIC cloning containing 
            N-terminal 6x His and MBP tags. C-terminal tags can be added during 
            SLIC..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Scholz J, Besir H, Strasser C, Suppmann S
  TITLE     A new method to customize protein expression vectors for fast, 
            efficient and background free parallel cloning.
  JOURNAL   BMC Biotechnol. 2013 Feb 14;13(1):12.
  PUBMED    23410102
REFERENCE   2  (bases 1 to 735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2013-02-14"; volume: "13(1)"; pages: "12"
COMMENT     Sequence Label: pCoofy49
FEATURES             Location/Qualifiers
     source          1..735
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..37)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(692..727)
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
ORIGIN
        1 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gctcattcca
       61 ggccctgggc gtcccagctc agggacacct tgttgttttc ggccttctgc acccagtcgc
      121 cattggcgtc cttggtgtgc tttctgccgg cctcgtccac ggccagttcg ctagaccgca
      181 cggacacgtc cacccgcagg ccgttggcgt ccatagcgtt gatgaactgg tggccgaagc
      241 ccttctgctt gtcgtcggac accaggctgc agcccacgat gctgatgtgg tcgggcttgt
      301 tgttgatgtt ctcggcctgg ttgaagctct gctggaactt ggccagcttc acggccagct
      361 cgtcggcgct gtagccgctc agccgggtgt tgtttgtctc gctgtggtcc ctgccgtgtc
      421 ccaccagctg ccaccgcagc ttgccgtcca gcttgctggg gtcgccgtac accacccggt
      481 agttgccgtc gctgtccagc tgcaccacca cgctgctctc ggcgtgcttg ccggccagat
      541 tggcagcggc cttggccacc acggggtcgt tttccatctg cacgatgatc tggccgtcga
      601 atcttgtctc gccgccgtcg gtggtagggg tcacggtgat ggggccccag ctgttcacgt
      661 tctggttgtg caggatcttg ccgctgcctc atttgccatc gatcagccgg gggtccacct
      721 ggtcctctca cagca
//
