LOCUS       sequence-91321-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces an active CBFD (carotenoid beta-ring 4-dehydrogenase) 
            enzyme in E. coli when expression is induced with arabinose.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Cunningham FX Jr, Gantt E
  TITLE     Elucidation of the pathway to astaxanthin in the flowers of Adonis 
            aestivalis.
  JOURNAL   Plant Cell. 2011 Aug;23(8):3055-69. doi: 10.1105/tpc.111.086827. 
            Epub 2011 Aug 23.
  PUBMED    21862704
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.111.086827"; journalName: "Plant Cell"; date: 
            "2011-08"; volume: "23"; issue: "8"; pages: "3055-69"
COMMENT     Sequence Label: pCBFD2Bad
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             52..74
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagaaataa ttttgtttaa
       61 ctttaagaag gagatataca tacccatggc agcagcaatt tcagtgttca gttcaggtta
      121 ttctttctac aagaatctct tgttggactc aaaaccaaat attctcaaac ccccatgcct
      181 gctattctct ccagttgtga tcatgtcgcc tatgagaaag aaaaagaaac atggtgatcc
      241 atgtatctgc tccgttgcag ggagaacaag gaaccttgat attcctcaaa ttgaagaaga
      301 ggaagagaat gtggaagaac taatagaaca gaccgattct gacatagtgc atataaagaa
      361 aacactaggg gggaaacaat caaaacggcc cactggctcc attgtcgcac ccgtatcttg
      421 tcttgggatc ctttcaatga ttggacctgc tgtttacttc aagttttcac ggctaatgga
      481 gggtggagat atacctgtag cagaaatggg gattacgttt gccacctttg ttgctgctgc
      541 tgttggcacg gagtttttgt cagcatgggt tcacaaagaa ctctggcacg agtctttgtg
      601 gtacattcac aagtctcacc atcggtcacg aaaaggccgc ttcgagttca atgatgtgtt
      661 tgctattatt aacgcgcttc ccgctattgc tcttatcaat tatggattct ccaatgaagg
      721 cctccttcct ggagcgtgct ttggtgtcgg tcttggaaca acagtctgtg gtatggctta
      781 catttttctt cacaatggcc tatcacaccg aaggttccca gtatggctta ttgcgaacgt
      841 cccttatttc cacaagctgg ctgcagctca ccaa
//
