LOCUS       sequence-91330-e         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains an Arabidopsis thaliana idi1 cDNA fused to a strong Trc 
            promoter. Produces an active isopentenyl diphosphate isomerase (IDI)
            in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Cunningham FX Jr, Lafond TP, Gantt E
  TITLE     Evidence of a role for LytB in the nonmevalonate pathway of 
            isoprenoid biosynthesis.
  JOURNAL   J Bacteriol. 2000 Oct;182(20):5841-8.
  PUBMED    11004185
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Bacteriol."; date: "2000-10"; volume: "182(20)"; pages: "5841-8"
COMMENT     Sequence Label: pAtipiTrc
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(860..886)
                     /codon_start=1
                     /product="synthetic cistron containing a ribosome binding 
                     site (Shine-Dalgarno sequence), for enhancing the bacterial
                     expression of a downstream cistron (Schoner, 1997)"
                     /label=minicistron
                     /note="This first cistron should terminate 3 bp upstream of
                     the ATG for the second cistron."
                     /translation="MYRLNKEE"
ORIGIN
        1 aactttatta ttgcctgagg tgcttggaag ttttgtaagt gtctcacata tcccaaatta
       61 ttatcatcga aaagataaaa atgatgaaga gaaagttgat tgatttaaaa actttaaaaa
      121 aagatgttca gagtttgtgg atggttttca tgtctatagc ttcaaccaaa gttcctttct
      181 caacatgatc ccaccacttc atcaagaaat tgtccaccac caatctgaac catggtgaca
      241 gtttcaaacc ttcctcacct gcatctgctt tcttcaccag ctccttcagc tcttcccggc
      301 tcacatactt gatctcagct acttcatctg ggtttggttg aaccttcacg tctcgcacga
      361 tgaagagcaa gtaatcaagt tcatgctctc cccatttgcc atcagaagga gccttgtaca
      421 gcatacgtcc caagggagtg aactcatcga ctggtacatc ttcagctaca ataccaagct
      481 catcgagaag ctttctttgt gcagcattcc tcacacctag tgcattgtcc tggataagct
      541 ctgattcacg gtaaagagga tggctgcaac aagtgttagt ccacactaga gggaacgtaa
      601 cctttgtgtt tgacctttgc tggagaagca actcatactt cgagttgaat aaaaatacac
      661 taaaagccct gtgcagcaaa ttcttggctt caatattttc catcagatga caattatact
      721 tgctgtcatg ccccacaaca cgatcagttt catcaacaag aatgcattca tcctcaaaca
      781 tgagacgtct ctgaacagca tccataccag catctttagt atctgtcata gcggtaccag
      841 ctgcagatct cgacatggtt tattcctcct tatttaatcg atacattaat atatacctct
      901 ttaatt
//
