LOCUS       sequence-91349-e        5235 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  JIP4 serves as an activator of the p38 mitogen-activated protein 
            (MAP) kinase pathway by a mechanism that requires the MAP kinase 
            kinases MKK3 and MKK6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5235)
  AUTHORS   Kelkar N, Standen CL, Davis RJ
  TITLE     Role of the JIP4 scaffold protein in the regulation of 
            mitogen-activated protein kinase signaling pathways.
  JOURNAL   Mol Cell Biol. 2005 Apr;25(7):2733-43.
  PUBMED    15767678
REFERENCE   2  (bases 1 to 5235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2005-04"; volume: "25(7)"; pages: "2733-43"
COMMENT     Sequence Label: pCDNA3 T7 Jip4
FEATURES             Location/Qualifiers
     source          1..5235
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        1..379
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        380..583
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     534..554
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     628..647
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        628..646
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             660..692
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     promoter        complement(4220..4238)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4221..4238)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4258..4275)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4264..4488
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4534..4962
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4621..4640)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4831..4852
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4971..4991)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
       61 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
      121 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      181 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      241 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      301 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      361 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      421 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      481 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      541 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga
      601 acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctta
      661 tggctagcat gactggtgga cagcaaatgg gtggatccat gatccataat tatatggaac
      721 acttagaaag aaccaaactt catcagctct cagggagtga tcaactagag gctacagctc
      781 atagtagaat tagaaaagaa cgtcctatat cattagggat tttccctcta cctgctggag
      841 atggattgct tacacctgac actcagaaag gaggcgagac cccaggatca gagcaatgga
      901 aatttcaaga attaagtcaa ccacgttctc ataccagcct gaaggatgaa ctttctgata
      961 ttagtcaagg tggatctaaa gctaccactc cagcttcaac agcaaattca gacgtatcag
     1021 caattcctcc tgatactccg tcaaaggaag ataatgaagg atttgtaaaa ggcacagata
     1081 catcaaataa gtcagagata agcaaacaca tagaagtcca ggttgcccaa gagactagga
     1141 atgtgtctac agaatctggt gaaaatgaag agaaatcaga agttcaagca atcattgaat
     1201 ccactcctga attggatatg gacaaagacc tcagtggata taaaggttca agcactccca
     1261 ccaaaggcat agagaacaaa gcttttgatc gcaatacaga atctctcttt gaagagctgt
     1321 cctcagctgg ctcgggcctt attggagatg tggatgaagg agcggattta ctaggaatgg
     1381 gtcgtgaggt ggagaatctc atactggaga acacacagct gctggaaacc aaaaacgctc
     1441 tgaatgtagt gaagaatgac ttaatagcca aagtggatga gctgacctgt gagaaggacg
     1501 tgctgcaggg ggagctggag gctgtgaagc aagccaagct gaagctggag gacaagaaca
     1561 gagagctgga ggaggagctc aggaaagctc gtgcagaagc tgaagatgca aggcaaaaag
     1621 caaaagacga tgatgatagt gacattccca cagcccagag gaagaggttt accagagtag
     1681 aaatggcccg agttctcatg gagagaaacc agtacaaaga gaggctgatg gagcttcaag
     1741 aagctgttcg atggacggag atgatccggg cctcacgaga gaacccagct atgcaagaaa
     1801 agaaaaggtc aagcatctgg caatttttca gccgactttt tagctcttca agtaatgcaa
     1861 caaagaagcc tgaaccccct gttaacctga agtacaacgc ccccacctct cacgtgactc
     1921 cctccgtgaa gaaaaggagc agcactttat ctcagctccc tggggacaag tccaaggcat
     1981 ttgatttcct cagtgaggaa actgaggcta gtttagcctc ccgcagagag caaaagagag
     2041 agcagtatcg gcaggtgaag gctcacgttc agaaggaaga tggccgagtg caagctttcg
     2101 gctggagtct gcctcagaag tacaaacagg tagccaatgg tcaaggggaa actaagatga
     2161 aaaatttacc tgtgcctgtc tatctcagac cactagacga aaaagatgcg tctatgaagc
     2221 tgtggtgtgc tgtaggagtc aatttatctg gtggcaagac aagagatggc gggtctgttg
     2281 ttggagcgag tgtattttac aaggatattg ctggtttgga cactgaaggc agtaaacagc
     2341 ggagtgcgtc tcagagtagt ttagacaagt tggatcaaga actcaaggaa cagcagaaag
     2401 aatttaaaaa tcaagaagag ttgtccagtc aggtctggat ctgtaccagc acccactcaa
     2461 ctacaaaggt tatcatcatt gatgctgttc agcctggcaa catcctagat agtttcactg
     2521 tttgcaattc tcatgttctg tgcattgcca gtgtcccagg agctcgggaa acagactacc
     2581 ctgcaggaga agaactttct gaatctggtc aggtagacaa agcgtcattg tgtggaagca
     2641 tgaccagtaa cagctccgca gagatggaca gcttgctggg aggcatcaca gtggttgggt
     2701 gctccacaga aggactgaca ggagctgcca cttccccaag taccaacggt gcttctcctg
     2761 tgattgaaaa gccaccagaa atggaaactg aaaatagcga agttgatgaa aatattccaa
     2821 cagcagaaga agcaactgaa gccacagagg gcaatgcagg gtccactgaa gacactgtgg
     2881 acatctccca gcctggcgtg tacacagagc atgtgtttac agatccactg ggagttcaga
     2941 tcccagaaga cctctcccca gtgtttcagt caagtaatga ctcagatgtg tacaaagatc
     3001 aaatatcagt attgccaaat gaacaagacc ttgcgagaga agaagctcag aaaatgagta
     3061 gtcttttacc aaccatgtgg cttggagctc agaatggctg tttgtatgtc cactcatctg
     3121 tagcccagtg gaggaaatgt ctccattcca ttaagctcaa agactcaatt ctcagtattg
     3181 tacatgtgaa aggaattgta ctagtggccc tggcggatgg cacccttgca atcttccaca
     3241 gaggagttga tggacaatgg gatctgtcaa actatcacct tttagacctt ggacgtcctc
     3301 atcattccat acgatgcatg actgtggtac atgacaaagt ctggtgtggc tataggaaca
     3361 aaatctatgt ggttcaacca aaggctatga agatagagaa atcatttgat gcacacccca
     3421 ggaaggagag ccaagtacgg cagcttgcat gggtgggtga tggtgtatgg gtctccattc
     3481 gtttggattc cacgctccgc ctctatcatg cacacacata ccaacatctc caggatgtgg
     3541 atattgagcc ttatgtaagc aaaatgttag gtactggaaa actgggtttc tctttcgtga
     3601 ggataacagc ccttatggtg tcttgcaatc gtttgtgggt ggggactgga aatggtgtca
     3661 ttatctccat cccactgaca gaaacaaata aaacctcagg gactccaggt aaccgtcctg
     3721 gaagtgtaat ccgtgtatat ggtgatgaga acagtgataa agtgactcca gggacattta
     3781 taccctactg ttcaatggca catgcacagc tttgtttcca cgggcaccgg gatgctgtga
     3841 aattctttgt ggcagtccca ggtcaggtca ttagcccaca aagtagcagt ggtggggcag
     3901 atcttacagc tgacaaggca gggtcatctg cacaggagcc cagtagccag acgcccttga
     3961 agtccatgct tgtcatcagt ggaggagagg gctacattga cttccggatg ggtgatgaag
     4021 gtggagaatc tgaacttctg ggagaggatc ttccactgga accttcagtc accaaagccg
     4081 agaggagtca cctgatagtg tggcaagtga tgtgtggcaa tgaatgagtc tgtgagagac
     4141 agacgaggat gaggaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aactcgagca
     4201 tgcatctaga gggccctatt ctatagtgtc acctaaatgc tagagctcgc tgatcagcct
     4261 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     4321 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     4381 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     4441 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     4501 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     4561 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     4621 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     4681 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     4741 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     4801 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     4861 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     4921 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     4981 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     5041 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     5101 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     5161 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgaataa ttttttttat
     5221 ttatgcaaaa ggccg
//
