LOCUS       sequence-91351-e         725 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tet-regulated (Tet-on) lentiviral vector for transgene (hUbiquitin 
            promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM 
            (Addgene#12247)) - 3rd generation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Szulc J, Wiznerowicz M, Sauvain MO, Trono D, Aebischer P
  TITLE     A versatile tool for conditional gene expression and knockdown.
  JOURNAL   Nat Methods. 2006 Feb;3(2):109-16. doi: 10.1038/nmeth846
  PUBMED    16432520
REFERENCE   2  (bases 1 to 725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Methods"; date: "2006-02"; volume: "3"; issue: "2"; pages: 
            "109-16"
COMMENT     Sequence Label: pLVUT-tTR-KRAB
FEATURES             Location/Qualifiers
     source          1..725
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(208..227)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt
       61 cgaggttaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac
      121 acgatgataa taccatggct agattagata aaagtaaagt gattaacagc gcattagagc
      181 tgcttaatga ggtcggaatc gaaggtttaa caacccgtaa actcgcccag aagctaggtg
      241 tagagcagcc tacattgtat tggcatgtaa aaaataagcg ggctttgctc gacgccttag
      301 ccattgagat gttagatagg caccatactc acttttgccc tttagaaggg gaaagctggc
      361 aagatttttt acgtaataac gctaaaagtt ttagatgtgc tttactaagt catcgcgatg
      421 gagcaaaagt acatttaggt acacggccta cagaaaaaca gtatgaaact ctcgaaaatc
      481 aattagcctt tttatgccaa caaggttttt cactagagaa tgcattatat gcactcagcg
      541 ctgtggggca ttttacttta ggttgcgtat tggaagatca agagcatcaa gtcgctaaag
      601 aagaaaggga aacacctact actgatagta tgccgccatt attacgacaa gctatcgaat
      661 tatttgatca ccaaggtgca gagccagcct tcttattcgg ccttgaattg atcatatgcg
      721 gatta
//
