LOCUS       sequence-91353-e         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tet-regulated (Tet-on) lentiviral vector for transgene (hUbiquitin 
            promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM 
            (Addgene#12247)) - 3rd generation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Szulc J, Wiznerowicz M, Sauvain MO, Trono D, Aebischer P
  TITLE     A versatile tool for conditional gene expression and knockdown.
  JOURNAL   Nat Methods. 2006 Feb;3(2):109-16. doi: 10.1038/nmeth846
  PUBMED    16432520
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Methods"; date: "2006-02"; volume: "3"; issue: "2"; pages: 
            "109-16"
COMMENT     Sequence Label: pLVUT-tTR-KRAB
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(219..238)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
ORIGIN
        1 ctcctcgccc ttgctcacca tggtggcgac cggtacccgg ggatcctcta gagtcgacct
       61 gcagcccaag cttcgtctaa caaaaaagcc aaaaacggcc agaatttagc ggacaattta
      121 ctagtctaac actgaaaatt acatattgac ccaaatgatt acatttcaaa aggtgcctaa
      181 aaaacttcac aaaacacact cgccaacccc gagcgcatag ttcaaaaccg gagcttcagc
      241 tacttaagaa gataggtaca taaaaccgac caaagaaact gacgcctcac ttatccctcc
      301 cctcaccaga ggtccggcgc ctgtcgattc aggagagcct accctaggcc cgaaccctgc
      361 gtcctgcgac ggagaaaagc ctaccgcaca cctaccggca ggtggcccca ccctgcatta
      421 taagccaaca gaacgggtga cgtcacgaca cgacgagggc gcgcgctccc aaaggtacgg
      481 gtgcactgcc caacggcacc gccataactg ccgcccccgc aacagacgac aaaccgagtt
      541 ctccagtcag tgacaaactt cacgtcaggg tccccagatg gtgccccagc ccatctcacc
      601 cgaataagag ctttcccgca ttagcgaagg cctcaagacc ttgggttctt gccgcccacc
      661 atgcccccca ccttgtttca acgacctcac agcccgcctc acaagcgtct tccattcaag
      721 actcgggaac agccgccatt ttgctgcgct ccccccaacc cccagttcag ggcaaccttg
      781 ctcgcgga
//
