LOCUS       sequence-91354-e         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tet-regulated (Tet-on) lentiviral vector for transgene (hUbiquitin 
            promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM 
            (Addgene#12247)) - 3rd generation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Szulc J, Wiznerowicz M, Sauvain MO, Trono D, Aebischer P
  TITLE     A versatile tool for conditional gene expression and knockdown.
  JOURNAL   Nat Methods. 2006 Feb;3(2):109-16. doi: 10.1038/nmeth846
  PUBMED    16432520
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Methods"; date: "2006-02"; volume: "3"; issue: "2"; pages: 
            "109-16"
COMMENT     Sequence Label: pLVUT-tTR-KRAB
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(165..359)
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     CDS             complement(462..482)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattcga agcttgagct
       61 cgactagcta gctagctact agctagtcga gatccttaaa ctgatgattt gatttcaaat
      121 gcagtctctg aatcaggatg ggtctcttgg tgaatttctc tctccaccag ccagggctct
      181 tctcccttct ccaaccggag gatcacatct ggcttagtaa gctgataacc caaggaaacc
      241 aggttcttat agttctccag catcacattt ctgtacacga tctgctgagc agtgtccagc
      301 agcttccact cctccctggt gaagtccaca aatacatcct tgaaggtcac cagtgtccgg
      361 gaccaggcag ttagtgactt agcatccatg ccctccttgt tcttgatgat acttccttga
      421 gtgacagcag agtgctgagg agacaaagca ccaccgccgt cgacctttct cttctttttt
      481 ggcgacccac tttcacattt aagttgtttt tctaatccgc atatgatcaa ttcaaggccg
      541 aataagaagg ctggctctgc accttggtga tcaaataatt cgatagcttg tcgtaataat
      601 ggcggcatac tatcagtagt aggtgtttcc ctttcttctt tagcgacttg atgctcttga
      661 tcttccaata cgcaacctaa agtaaaatgc cccacagcgc tgagtgcata taatgcattc
      721 tctagtgaaa aaccttgttg gcataaaaag gctaattgat tttcgagagt ttcatactgt
      781 ttttc
//
