LOCUS       sequence-91364-e         952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vivo visualization of the endoplasmic reticulum (can be used for 
            colocalization studies).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Goedhart J, von Stetten D, Noirclerc-Savoye M, Lelimousin M, Joosen 
            L, Hink MA, van Weeren L, Gadella TW Jr, Royant A
  TITLE     Structure-guided evolution of cyan fluorescent proteins towards a 
            quantum yield of 93%.
  JOURNAL   Nat Commun. 2012 Mar 20;3:751. doi: 10.1038/ncomms1738.
  PUBMED    22434194
REFERENCE   2  (bases 1 to 952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012-03-20"; volume: "3:751. doi"; pages: " 
            10.1038/ncomms1738"
COMMENT     Sequence Label: pmTurquoise2-ER
FEATURES             Location/Qualifiers
     source          1..952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        88..192
                     /gene="bla"
                     /label=AmpR promoter
     promoter        194..551
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        194..385
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(217..237)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      402..537
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(640..659)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
       61 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
      121 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
      181 aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg
      241 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
      301 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
      361 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
      421 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
      481 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
      541 gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
      601 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
      661 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
      721 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg
      781 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
      841 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
      901 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gc
//
