LOCUS       sequence-91370-e         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vivo visualization of the endoplasmic reticulum (can be used for 
            colocalization studies).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Goedhart J, von Stetten D, Noirclerc-Savoye M, Lelimousin M, Joosen 
            L, Hink MA, van Weeren L, Gadella TW Jr, Royant A
  TITLE     Structure-guided evolution of cyan fluorescent proteins towards a 
            quantum yield of 93%.
  JOURNAL   Nat Commun. 2012 Mar 20;3:751. doi: 10.1038/ncomms1738.
  PUBMED    22434194
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012-03-20"; volume: "3:751. doi"; pages: " 
            10.1038/ncomms1738"
COMMENT     Sequence Label: pmTurquoise2-ER
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     84..137
                     /codon_start=1
                     /product="signal sequence of chicken lysozyme"
                     /label=lysozyme signal sequence
                     /translation="MRSLLILVLCFLPLAALG"
     regulatory      186..195
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(192..194,195..197,198..710)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(237..258)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(498..517)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     845..866
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ttagcagagc tggtttagtg aaccgtcaga tccgctagcg ctaccggact cagatctcga
       61 gctcaagctt cgaattcgcc accatgaggt ctttgctaat cttggtgctt tgcttcctgc
      121 ccctggctgc tctggggaaa gtctttggac gatgtgagct ggcagcggct atgaagccac
      181 cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
      241 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
      301 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
      361 cctggcccac cctcgtgacc accctgtcct ggggcgtgca gtgcttcgcc cgctaccccg
      421 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
      481 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
      541 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
      601 tcctggggca caagctggag tacaactact ttagcgacaa cgtctatatc accgccgaca
      661 agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag gacggcggcg
      721 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
      781 ccgacaacca ctacctgagc acccagtcca agctgagcaa agaccccaac gagaagcgcg
      841 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc a
//
