LOCUS       sequence-91393-e        1029 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependent activation of gene of interest by removing Stop 
            sequences; Puro selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Jackson EL, Willis N, Mercer K, Bronson RT, Crowley D, Montoya R, 
            Jacks T, Tuveson DA
  TITLE     Analysis of lung tumor initiation and progression using conditional 
            expression of oncogenic K-ras.
  JOURNAL   Genes Dev. 2001 Dec 15. 15(24):3243-8.
  PUBMED    11751630
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2001 Dec 15. 15(24):3243-8."
COMMENT     Sequence Label: Lox-Stop-Lox TOPO
FEATURES             Location/Qualifiers
     source          1..1029
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        21..39
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     21..38
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    141..174
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      205..214
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    239..360
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(250..269)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     304..323
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(785..805)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          935..1000
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 gaccatgatt acgccaagct atttaggtga cactatagaa tactcaagct atgcatcaag
       61 cttggtacca actcgagtta attaaccacg cgtggcgcgc cttgagctcg gatccgggcc
      121 ccccctcgat cgaggtcgac ataacttcgt ataatgtatg ctatacgaag ttattaggtc
      181 cctcgacctg cagacttact caagccatgg tggctagctt gcggaattcc agacatgata
      241 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
      301 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
      361 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
      421 taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatctcta gtcaaggcac
      481 tatacatcaa atattcctta ttaacccctt tacaaattaa aaagctaaag gtacacaatt
      541 tttgagcata gttattaata gcagacactc tatgcctgtg tggagtaaga aaaaacagta
      601 tgttatgatt ataactgtta tgcctactta taaaggttac agaatatttt tccataattt
      661 tcttgtatag cagtgcagct ttttcctttg tggtgtaaat agcaaagcaa gcaagagttc
      721 tattactaaa cacagcatga ctcaaaaaac ttagcaattc tgaaggaaag tccttggggt
      781 cttctacctt tctcttcttt tttggaggag tagaatgttg agagtcagca gtagcctcat
      841 catcactaga tggcatttct tctgagcaaa acaggttttc ctcattaaag gcattccacc
      901 actgctccca ttcatcagtt ccataggttg gaatctaaaa tacacaaaca attagaatca
      961 gtagtttaac acattataca cttaaaaatt ttatatttac cttagagctt taaatctctg
     1021 taggtagtt
//
