LOCUS       sequence-91396-e         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependent activation of gene of interest by removing Stop 
            sequences; Puro selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Jackson EL, Willis N, Mercer K, Bronson RT, Crowley D, Montoya R, 
            Jacks T, Tuveson DA
  TITLE     Analysis of lung tumor initiation and progression using conditional 
            expression of oncogenic K-ras.
  JOURNAL   Genes Dev. 2001 Dec 15. 15(24):3243-8.
  PUBMED    11751630
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2001 Dec 15. 15(24):3243-8."
COMMENT     Sequence Label: Lox-Stop-Lox TOPO
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          605..670
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             800..820
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aatttcgagg tcgaagggcg cagtagtcca gggtttcctt gatgatgtca tacttatcct
       61 gtcccttttt tttccacagg gatcctcggg gacaccaaat atggcgatct cggccttttc
      121 gtttcttgga gctgggacat gtttgccatc gatccatcta ccaccagaac ggccgttaga
      181 tctgctgcca ccgttgtttc caccgaagaa accaccgttg ccgtaaccac cacgacggtt
      241 gttgctaaag aagctgccac cgccacggcc accgttgtag ccgccgttgt tgttattgta
      301 gttgctactg ttatttctgg cacttcttgg ttttcctctt aagtgaggag gaacataacc
      361 attctcgttg ttgtcgttga tgcttaaatt ttgcacttgt tcgctcagtt cagccataat
      421 atgaaatgct tttcttgttg ttcttacgga ataccacttg ccacctatca ccacaactaa
      481 ctttttcccg ttcctccatc tcttttatat tttttttctc gatcgaggga tctttgtgaa
      541 ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc
      601 taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg
      661 tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa
      721 tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga
      781 ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc
      841 ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt
      901 tgctatttac accacaaagg aaaaa
//
