LOCUS       sequence-91409-e         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  can be used to monitor the ATP:ADP ratio during live-cell imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Berg J, Hung YP, Yellen G
  TITLE     A genetically encoded fluorescent reporter of ATP:ADP ratio.
  JOURNAL   Nat Methods. 2009 Feb;6(2):161-6. doi: 10.1038/nmeth.1288.
  PUBMED    19122669
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2009-02"; volume: "6"; issue: 
            "2"; pages: "161-6"
COMMENT     Sequence Label: GW1CMV-Perceval
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             528..779
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSFQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     716..737
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(840..861)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ctcccgttgc ggtgctgtta acggtggagg gcagtgtagt ctgagcagta ctcgttgctg
       61 ccgcgcgcgc caccagacat aatagctgac agactaacag actgttcctt tccatgggtc
      121 ttttctgcag tcaccgtcct tgacacgaag cttcccggcc acagcaatcg atccatgaca
      181 caaccagtga caagcagtcc cccagtgaaa ccgcatccgc atccgagtcc gataccgata
      241 aagattctga atcggagtga gtgccgcgtc cgagagcgtt ccctgtccac gtccaccatc
      301 ggcggagcag gtgtgcctga ggcctctaga ccaccatgaa aaaggttgaa tccatcatca
      361 ggcccgaaaa gctggagatc gttaagaagg ctctctcgga cgctggatat gtgggtatga
      421 cagtctctga ggtcaagggc acgggcgtcc agggcggcat cgtcgagagg taccgaggaa
      481 gggagtactc tgcaggctac aacagcgaca acgtctatat caccgccgac aagcagaaga
      541 acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcggc gtgcagctcg
      601 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
      661 actacctgag cttccagtcc aagctgagca aagaccccaa cgagaagcgc gatcacatgg
      721 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg
      781 gcggttccgg aggcatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
      841 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc g
//
