LOCUS       sequence-91435-e         650 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expresses HA tagged channelrhodopsin and hM4D (Gi) from a 
            Cre-dependent virus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Stachniak TJ, Ghosh A, Sternson SM
  TITLE     Chemogenetic synaptic silencing of neural circuits localizes a 
            hypothalamus-->midbrain pathway for feeding behavior.
  JOURNAL   Neuron. 2014 May 21;82(4):797-808. doi: 
            10.1016/j.neuron.2014.04.008. Epub 2014 Apr 24.
  PUBMED    24768300
REFERENCE   2  (bases 1 to 650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2014.04.008"; journalName: "Neuron"; date: 
            "2014-05-21- 21"; volume: "82"; issue: "4"; pages: "797-808"
COMMENT     Sequence Label: AAV-CAG::FLEX-rev:: ChR2HA-2a-hM4D
FEATURES             Location/Qualifiers
     source          1..650
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..73
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             47..73
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             74..100
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             107..160
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 ggtcgagact ctcgtcgaag acgaagccga ggccggagcc gtggcatacc cctacgacgt
       61 gcccgactac gcctatccat atgatgtccc agactatgct gctagcgaag gacgtggatc
      121 tctgctgacc tgtggagatg tggaagagaa tcccggacca tcgcgaatgg ccaacttcac
      181 acctgtcaat ggcagctcgg gcaatcagtc cgtgcgcctg gtcacgtcat catcccacaa
      241 tcgctatgag acggtggaaa tggtcttcat tgccacagtg acaggctccc tgagcctggt
      301 gactgtcgtg ggcaacatcc tggtgatgct gtccatcaag gtcaacaggc agctgcagac
      361 agtcaacaac tacttcctct tcagcctggc gtgtgctgat ctcatcatag gcgccttctc
      421 catgaacctc tacaccgtgt acatcatcaa gggctactgg cccctgggcg ccgtggtctg
      481 cgacctgtgg ctggccctgg actgcgtggt gagcaacgcc tccgtcatga accttctcat
      541 catcagcttt gaccgctact tctgcgtcac caagcctctc acctaccctg cccggcgcac
      601 caccaagatg gcaggcctca tgattgctgc tgcctgggta ctgtccttcg
//
