LOCUS       sequence-91441-e         568 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pUC cloning vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Norrander J, Kempe T, Messing J
  TITLE     Construction of improved M13 vectors using 
            oligodeoxynucleotide-directed mutagenesis.
  JOURNAL   Gene. 1983 Dec;26(1):101-6.
  PUBMED    6323249
REFERENCE   2  (bases 1 to 568)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "1983-12"; volume: "26(1)"; pages: "101-6"
COMMENT     Sequence Label: pUC12
FEATURES             Location/Qualifiers
     source          1..568
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(41..58)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(41..57)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(50..72)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(266..285)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     385..407
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(445..463)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ccggggatcc tctagagtcg acctgcagcc caagcttggc actggccgtc gttttacaac
       61 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
      121 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
      181 gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
      241 cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
      301 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
      361 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
      421 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
      481 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
      541 ctatttgttt atttttctaa atacattc
//
