LOCUS       sequence-91445-e         413 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pUC cloning vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Norrander J, Kempe T, Messing J
  TITLE     Construction of improved M13 vectors using 
            oligodeoxynucleotide-directed mutagenesis.
  JOURNAL   Gene. 1983 Dec;26(1):101-6.
  PUBMED    6323249
REFERENCE   2  (bases 1 to 413)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "1983-12"; volume: "26(1)"; pages: "101-6"
COMMENT     Sequence Label: pUC18
FEATURES             Location/Qualifiers
     source          1..413
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(27..44)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(27..43)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(36..58)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(252..271)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     371..393
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 tcgacctgca ggcatgcaag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa
       61 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
      121 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
      181 tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
      241 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
      301 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
      361 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaa
//
