LOCUS       sequence-91449-e         672 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pUC cloning vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Norrander J, Kempe T, Messing J
  TITLE     Construction of improved M13 vectors using 
            oligodeoxynucleotide-directed mutagenesis.
  JOURNAL   Gene. 1983 Dec;26(1):101-6.
  PUBMED    6323249
REFERENCE   2  (bases 1 to 672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "1983-12"; volume: "26(1)"; pages: "101-6"
COMMENT     Sequence Label: pUC19
FEATURES             Location/Qualifiers
     source          1..672
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(49..66)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(49..65)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(58..80)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(274..293)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     393..415
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(453..471)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        539..643
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgcctgcagg tcgactctag aggatccccg ggtaccgagc tcgaattcac tggccgtcgt
       61 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
      121 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
      181 gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg
      241 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
      301 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
      361 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
      421 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
      481 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
      541 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
      601 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
      661 ccgtgtcgcc ct
//
