LOCUS       sequence-91450-e         658 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pUC cloning vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Timmermans MC, Maliga P, Vieira J, Messing J
  TITLE     The pFF plasmids: cassettes utilising CaMV sequences for expression 
            of foreign genes in plants.
  JOURNAL   J Biotechnol. 1990 Jun;14(3-4):333-44.
  PUBMED    1369289
REFERENCE   2  (bases 1 to 658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Biotechnol."; date: "1990-06"; volume: "14(3-4)"; pages: "333-44"
COMMENT     Sequence Label: pUC120
FEATURES             Location/Qualifiers
     source          1..658
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(43..60)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(43..59)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(52..74)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      292..650
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(379..398)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     589..610
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cggggatcct ctagagtcga cctgcaggca tgcaagcttg gcactggccg tcgttttaca
       61 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
      121 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
      181 cagcctgcct aatgagcggg cttttttttt ggcgaatggc gcctgatgcg gtattttctc
      241 cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct
      301 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
      361 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
      421 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
      481 ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct
      541 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
      601 tccaaactgg aacaacactc aaccctatct cgggctattc ttttgattta taagggat
//
