LOCUS       sequence-91452-e         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli cloning vector (KanR, high copy, blue/white selection, M13 
            IR).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Vieira J, Messing J
  TITLE     New pUC-derived cloning vectors with different selectable markers 
            and DNA replication origins.
  JOURNAL   Gene. 1991 Apr;100:189-94.
  PUBMED    1905257
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "1991-04"; volume: "100"; pages: "189-94"
COMMENT     Sequence Label: pUK21
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(21..37)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(21..37)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(165..181)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(165..181)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(178..200)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    189..205
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    260..281
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(398..415)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(649..668)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tatagggcga attggggatc gatccactag ttctagagcg gccgccacgg cgatatcgga
       61 tccatatgac gtcgacgcgt ctgcagaagc ttcgaattcg agctcccggg taccatggca
      121 tgcatcgata gatctcgagg cctcggacta gtggcgtaat catggtcata gctgtttcct
      181 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccgcgga agcataaagt
      241 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
      301 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
      361 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
      421 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
      481 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
      541 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
      601 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
      661 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
      721 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgct
//
