LOCUS       sequence-91479-e        1699 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  JIP4 serves as an activator of the p38 mitogen-activated protein 
            (MAP) kinase pathway by a mechanism that requires the MAP kinase 
            kinases MKK3 and MKK6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Kelkar N, Standen CL, Davis RJ
  TITLE     Role of the JIP4 scaffold protein in the regulation of 
            mitogen-activated protein kinase signaling pathways.
  JOURNAL   Mol Cell Biol. 2005 Apr;25(7):2733-43.
  PUBMED    15767678
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2005-04"; volume: "25(7)"; pages: "2733-43"
COMMENT     Sequence Label: pCDNA3 T7 Jip4 (1-151)
FEATURES             Location/Qualifiers
     source          1..1699
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        10..314
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        315..518
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     469..489
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     563..582
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        563..581
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             595..627
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     promoter        complement(1124..1142)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1125..1142)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1162..1179)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1168..1392
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(1525..1544)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
       61 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      121 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      181 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      241 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      301 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      361 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      421 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      481 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      541 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttatggct
      601 agcatgactg gtggacagca aatgggtgga tccatgatcc ataattatat ggaacactta
      661 gaaagaacca aacttcatca gctctcaggg agtgatcaac tagaggctac agctcatagt
      721 agaattagaa aagaacgtcc tatatcatta gggattttcc ctctacctgc tggagatgga
      781 ttgcttacac ctgacactca gaaaggaggc gagaccccag gatcagagca atggaaattt
      841 caagaattaa gtcaaccacg ttctcatacc agcctgaagg atgaactttc tgatattagt
      901 caaggtggat ctaaagctac cactccagct tcaacagcaa attcagacgt atcagcaatt
      961 cctcctgata ctccgtcaaa ggaagataat gaaggatttg taaaaggcac agatacatca
     1021 aataagtcag agataagcaa acacatagaa gtccaggttg cccaagagac taggaatgtg
     1081 tctacatgag cggccgctcg agcatgcatc tagagggccc tattctatag tgtcacctaa
     1141 atgctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     1201 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     1261 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     1321 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     1381 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     1441 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     1501 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     1561 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     1621 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     1681 cgccctgata gacggtttt
//
