LOCUS       sequence-91480-e        1453 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  JIP4 serves as an activator of the p38 mitogen-activated protein 
            (MAP) kinase pathway by a mechanism that requires the MAP kinase 
            kinases MKK3 and MKK6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Kelkar N, Standen CL, Davis RJ
  TITLE     Role of the JIP4 scaffold protein in the regulation of 
            mitogen-activated protein kinase signaling pathways.
  JOURNAL   Mol Cell Biol. 2005 Apr;25(7):2733-43.
  PUBMED    15767678
REFERENCE   2  (bases 1 to 1453)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2005-04"; volume: "25(7)"; pages: "2733-43"
COMMENT     Sequence Label: pCDNA3 T7 Jip4 (1-212)
FEATURES             Location/Qualifiers
     source          1..1453
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        113..316
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     267..287
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     361..380
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        361..379
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             393..425
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     promoter        complement(1105..1123)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1106..1123)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1143..1160)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1149..1374
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
       61 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
      121 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
      181 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
      241 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
      301 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
      361 taatacgact cactataggg agacccaagc ttatggctag catgactggt ggacagcaaa
      421 tgggtggatc catgatccat aattatatgg aacacttaga aagaaccaaa cttcatcagc
      481 tctcagggag tgatcaacta gaggctacag ctcatagtag aattagaaaa gaacgtccta
      541 tatcattagg gattttccct ctacctgctg gagatggatt gcttacacct gacactcaga
      601 aaggaggcga gaccccagga tcagagcaat ggaaatttca agaattaagt caaccacgtt
      661 ctcataccag cctgaaggat gaactttctg atattagtca aggtggatct aaagctacca
      721 ctccagcttc aacagcaaat tcagacgtat cagcaattcc tcctgatact ccgtcaaagg
      781 aagataatga aggatttgta aaaggcacag atacatcaaa taagtcagag ataagcaaac
      841 acatagaagt ccaggttgcc caagagacta ggaatgtgtc tacagaatct ggtgaaaatg
      901 aagagaaatc agaagttcaa gcaatcattg aatccactcc tgaattggat atggacaaag
      961 acctcagtgg atataaaggt tcaagcactc ccaccaaagg catagagaac aaagcttttg
     1021 atcgcaatac agaatctctc tttgaagagc tgtcctcagc tggctcgtga gcggccgctc
     1081 gagcatgcat ctagagggcc ctattctata gtgtcaccta aatgctagag ctcgctgatc
     1141 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     1201 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     1261 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     1321 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     1381 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
     1441 taagcgcggc ggg
//
