LOCUS       sequence-91481-e        1292 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  JIP4 serves as an activator of the p38 mitogen-activated protein 
            (MAP) kinase pathway by a mechanism that requires the MAP kinase 
            kinases MKK3 and MKK6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Kelkar N, Standen CL, Davis RJ
  TITLE     Role of the JIP4 scaffold protein in the regulation of 
            mitogen-activated protein kinase signaling pathways.
  JOURNAL   Mol Cell Biol. 2005 Apr;25(7):2733-43.
  PUBMED    15767678
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2005-04"; volume: "25(7)"; pages: "2733-43"
COMMENT     Sequence Label: pCDNA3 T7 Jip4 (1-272)
FEATURES             Location/Qualifiers
     source          1..1292
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        34..133
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     84..104
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     178..197
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        178..196
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             210..242
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     promoter        complement(1102..1120)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1103..1120)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1140..1157)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1146..1257
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
       61 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
      121 tataagcaga gctctctggc taactagaga acccactgct tactggctta tcgaaattaa
      181 tacgactcac tatagggaga cccaagctta tggctagcat gactggtgga cagcaaatgg
      241 gtggatccat gatccataat tatatggaac acttagaaag aaccaaactt catcagctct
      301 cagggagtga tcaactagag gctacagctc atagtagaat tagaaaagaa cgtcctatat
      361 cattagggat tttccctcta cctgctggag atggattgct tacacctgac actcagaaag
      421 gaggcgagac cccaggatca gagcaatgga aatttcaaga attaagtcaa ccacgttctc
      481 ataccagcct gaaggatgaa ctttctgata ttagtcaagg tggatctaaa gctaccactc
      541 cagcttcaac agcaaattca gacgtatcag caattcctcc tgatactccg tcaaaggaag
      601 ataatgaagg atttgtaaaa ggcacagata catcaaataa gtcagagata agcaaacaca
      661 tagaagtcca ggttgcccaa gagactagga atgtgtctac agaatctggt gaaaatgaag
      721 agaaatcaga agttcaagca atcattgaat ccactcctga attggatatg gacaaagacc
      781 tcagtggata taaaggttca agcactccca ccaaaggcat agagaacaaa gcttttgatc
      841 gcaatacaga atctctcttt gaagagctgt cctcagctgg ctcgggcctt attggagatg
      901 tggatgaagg agcggattta ctaggaatgg gtcgtgaggt ggagaatctc atactggaga
      961 acacacagct gctggaaacc aaaaacgctc tgaatgtagt gaagaatgac ttaatagcca
     1021 aagtggatga gctgacctgt gagaaggacg tgctgcaggg ggagtgagcg gccgctcgag
     1081 catgcatcta gagggcccta ttctatagtg tcacctaaat gctagagctc gctgatcagc
     1141 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     1201 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     1261 ttgtctgagt aggtgtcatt ctattctggg gg
//
